Diamond Publications - Search Results

Search Again...

These results include publications that have not yet been reviewed and processed by Diamond Light Source. To view only processed publications click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
B21-High Throughput SAXS	Interaction of the gtpase elongation factor Like-1 with the Shwachman-Diamond Syndrome protein and its missense mutations Abril Gijsbers , Diana Montagut , Alfonso Méndez-Godoy , Davide Altamura , Michele Saviano , Dritan Siliqi , Nuria Sánchez-Puig International Journal Of Molecular Sciences 19 DOI: 10.3390/ijms19124012 Diamond Proposal Number(s): [15832] Open Access Show Abstract ▼ Abstract: The Shwachman-Diamond Syndrome (SDS) is a disorder arising from mutations in the genes encoding for the Shwachman-Bodian-Diamond Syndrome (SBDS) protein and the GTPase known as Elongation Factor Like-1 (EFL1). Together, these proteins remove the anti-association factor eIF6 from the surface of the pre-60S ribosomal subunit to promote the formation of mature ribosomes. SBDS missense mutations can either destabilize the protein fold or affect surface epitopes. The molecular alterations resulting from the latter remain largely unknown, although some evidence suggest that binding to EFL1 may be affected. We further explored the effect of these SBDS mutations on the interaction with EFL1, and showed that all tested mutations disrupted the binding to EFL1. Binding was either severely weakened or almost abolished, depending on the assessed mutation. In higher eukaryotes, SBDS is essential for development, and lack of the protein results in early lethality. The existence of patients whose only source of SBDS consists of that with surface missense mutations highlights the importance of the interaction with EFL1 for their function. Additionally, we studied the interaction mechanism of the proteins in solution and demonstrated that binding consists of two independent and cooperative events, with domains 2–3 of SBDS directing the initial interaction with EFL1, followed by docking of domain 1. In solution, both proteins exhibited large flexibility and consisted of an ensemble of conformations, as demonstrated by Small Angle X-ray Scattering (SAXS) experiments.	Dec 2018

Instruments / Technical Area

Publication Details

Published

Actions

B21-High Throughput SAXS

Interaction of the gtpase elongation factor Like-1 with the Shwachman-Diamond Syndrome protein and its missense mutations

Abril Gijsbers , Diana Montagut , Alfonso Méndez-Godoy , Davide Altamura , Michele Saviano , Dritan Siliqi , Nuria Sánchez-Puig

International Journal Of Molecular Sciences 19

DOI: 10.3390/ijms19124012

Diamond Proposal Number(s): [15832]