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Instruments / Technical Area	Publication Details	Published	Actions
I02-Macromolecular Crystallography I03-Macromolecular Crystallography	Crystal structures of the two domains that constitute the Plasmodium vivax p43 protein Swati Gupta , Jyoti Chhibber-Goel , Manmohan Sharma , Suhel Parvez , Karl Harlos , Amit Sharma , Manickam Yogavel Acta Crystallographica Section D Structural Biology 76 DOI: 10.1107/S2059798319016413 Diamond Proposal Number(s): [14744] Show Abstract ▼ Abstract: Scaffold modules known as aminoacyl-tRNA synthetase (aaRS)-interacting multifunctional proteins (AIMPs), such as AIMP1/p43, AIMP2/p38 and AIMP3/p18, are important in driving the assembly of multi-aaRS (MARS) complexes in eukaryotes. Often, AIMPs contain an N-terminal glutathione S-transferase (GST)-like domain and a C-terminal OB-fold tRNA-binding domain. Recently, the apicomplexan-specific Plasmodium falciparum p43 protein (Pfp43) has been annotated as an AIMP and its tRNA binding, tRNA import and membrane association have been characterized. The crystal structures of both the N- and C-terminal domains of the Plasmodium vivax p43 protein (Pvp43), which is an ortholog of Pfp43, have been resolved. Analyses reveal the overall oligomeric structure of Pvp43 and highlight several notable features that show Pvp43 to be a soluble, cytosolic protein. The dimeric assembly of the N-terminal GST-like domain of Pvp43 differs significantly from canonical GST dimers, and it is tied to the C-terminal tRNA-binding domain via a linker region. This work therefore establishes a framework for dissecting the additional roles of p43 orthologs in eukaryotic multi-protein MARS complexes.	Feb 2020

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I02-Macromolecular Crystallography
I03-Macromolecular Crystallography

Crystal structures of the two domains that constitute the Plasmodium vivax p43 protein

Swati Gupta , Jyoti Chhibber-Goel , Manmohan Sharma , Suhel Parvez , Karl Harlos , Amit Sharma , Manickam Yogavel

Acta Crystallographica Section D Structural Biology 76

DOI: 10.1107/S2059798319016413

Diamond Proposal Number(s): [14744]

Show Abstract ▼

Feb 2020