Search Results

You searched for all publications:

with publication states 'Published (Approved)'

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

Exact matches
Related results

14,332 items found (0 items not approved), displaying 13,911 to 13,920.[First/Prev] 1,388, 1,389, 1,390, 1,391, 1,392, 1,393, 1,394, 1,395 [Next/Last]

Instruments / Technical Area	Publication Details	Published
NONE-No attached Diamond beamline	Thioredoxin A active-site mutants form mixed disulfide dimers that resemble enzyme-substrate reaction intermediates. Thijs R. H. M. Kouwen , Rianne Schrijver , Jean-Yves F. Dubois , Megan J. Maher , Elizabeth P. Carpenter , Juni Andrell , So Iwata , Jan Maarten Van Dijl Journal Of Molecular Biology 379 (3), 520-34 DOI: 10.1016/j.jmb.2008.03.077 PMID: 18455736 Open Access Show Abstract ▼ Abstract: Thioredoxin functions in nearly all organisms as the major thiol-disulfide oxidoreductase within the cytosol. Its prime purpose is to maintain cysteine-containing proteins in the reduced state by converting intramolecular disulfide bonds into dithiols in a disulfide exchange reaction. Thioredoxin has been reported to contribute to a wide variety of physiological functions by interacting with specific sets of substrates in different cell types. To investigate the function of the essential thioredoxin A (TrxA) in the low-GC Gram-positive bacterium Bacillus subtilis, we purified wild-type TrxA and three mutant TrxA proteins that lack either one or both of the two cysteine residues in the CxxC active site. The pure proteins were used for substrate-binding studies known as "mixed disulfide fishing" in which covalent disulfide-bonded reaction intermediates can be visualized. An unprecedented finding is that both active-site cysteine residues can form mixed disulfides with substrate proteins when the other active-site cysteine is absent, but only the N-terminal active-site cysteine forms stable interactions. A second novelty is that both single-cysteine mutant TrxA proteins form stable homodimers due to thiol oxidation of the remaining active-site cysteine residue. To investigate whether these dimers resemble mixed enzyme-substrate disulfides, the structure of the most abundant dimer, C32S, was characterized by X-ray crystallography. This yielded a high-resolution (1.5A) X-ray crystallographic structure of a thioredoxin homodimer from a low-GC Gram-positive bacterium. The C32S TrxA dimer can be regarded as a mixed disulfide reaction intermediate of thioredoxin, which reveals the diversity of thioredoxin/substrate-binding modes.	Jun 2008
NONE-No attached Diamond beamline	Molecular mechanism of energy conservation in polysulfide respiration M. Jormakka , K. Yokoyama , S. Yano , M. Tamakoshi , S. Akimoto , T. Shimamura , P. Curmi , S. Iwata Nature Structural & Molecular Biology 15 (7), 730 - 737 DOI: 10.1038/nsmb.1434 Show Abstract ▼ Abstract: Bacterial polysulfide reductase (PsrABC) is an integral membrane protein complex responsible for quinone-coupled reduction of polysulfide, a process important in extreme environments such as deep-sea vents and hot springs. We determined the structure of polysulfide reductase from Thermus thermophilus at 2.4-Å resolution, revealing how the PsrA subunit recognizes and reduces its unique polyanionic substrate. The integral membrane subunit PsrC was characterized using the natural substrate menaquinone-7 and inhibitors, providing a comprehensive representation of a quinone binding site and revealing the presence of a water-filled cavity connecting the quinone binding site on the periplasmic side to the cytoplasm. These results suggest that polysulfide reductase could be a key energy-conserving enzyme of the T. thermophilus respiratory chain, using polysulfide as the terminal electron acceptor and pumping protons across the membrane via a previously unknown mechanism.	Jun 2008
Accelerator Physics	Performances and Trends of Storage Ring Light Sources Riccardo Bartolini 11th European Particle Accelerator Conference Show Abstract ▼ Abstract: We present an overview of the performance of the latest generation of operating storage ring light sources. Emphasis is given to the comparison of design parameters to the achieved performances. Trends and innovations of established light sources to meet the increasing user's demand for high brilliance and different time structures will be presented. Report on upgrades and improvements will be given including orbit stability, top-up, feedback systems, lower-ID gap operation and a review of the activities for the generation of ultra-short radiation pulses in storage rings.	Jun 2008
Accelerator Physics	Top-Up Safety Simulations for the Diamond Storage Ring C. P. Bailey , R. Bartolini , E. C. Longhi , R. P. Walker , I. P. S. Martin 11th European Particle Accelerator Conference (EPAC08) Show Abstract ▼ Abstract: To ensure that it is not possible for a train of injected electron bunches to pass down the open beam-line during top-up operation at the Diamond Light Source, an extensive program of tracking studies has been performed. various error scenarios have been investigated, with realistic magnetic field, trajectory, aperture and energy errors all take into account. We describe teh tracking methods used, scenarios considered and the interlocks required in order to maintain safety during top-up operation.	Jun 2008
Accelerator Physics	Further Advances in Understanding and Optimising Beam Dynamics in the Diamond Storage Ring Riccardo Bartolini , Richard Fielder , Emily Longhi , Beni Singh , Ian Martin 11th European Particle Accelerator Conference	Jun 2008
	First Year's Experience of Diamond's Insertion Devices Emily Longhi , Riccardo Bartolini , Richard Fielder 11th European Parcticle Accelerator Conference	Jun 2008
Mechanical Engineering	Assessment of the Impact of External Stimuli on the Floor Stability of Diamond James Kay , Houcheng Huang , Riccardo Bartolini EPAC 2008 Show Abstract ▼ Abstract: Continuous vibration monitoring is carried out and the stability of the Diamond floor slab has been assessed with regard to how it has responded to various external stimuli. Data has been collected on weather conditions and comparisons made at extremes with floor vibration. The impact of a high level walkway bridge on the hall floor has also been assessed and there was a unique opportunity for an operational facility to measure the vibration response during a complete power blackout. The impact of local construction work is also presented.	Jun 2008
Accelerator Physics Mechanical Engineering	Analysis of beam Orbit stability and Ground vibrations at the diamond storage ring Riccardo Bartolini , Houcheng Huang , James Kay , I Martin EPAC 2008 Show Abstract ▼ Abstract: With the aim of understanding and improving the beam orbit stability at the Diamond storage ring we launched an extensive campaign of ground and magnet vibration measurements in order to identify the sources of ground vibration and how they affect the beam orbit stability through the girder resonances. We present here the results of the measurements performed along with a discussion of the possible remedies and the implications for the orbit feedback systems.	Jun 2008
Accelerator Physics	SAPPHIRE A high peak brightness x-ray source as a possible option for a next generation UK light source R. Bartolini , C. Christou , J. H. Han , J. Kay , R. Walker EPAC08 proceedings Show Abstract ▼ Abstract: We present the results of initial studies of one option for a next generation UK light source, based on a normal conducting S-band linac and photocathode gun.	Jun 2008
Mechanical Engineering	First Results from a Hydrostatic Levelling System Installed at Diamond Light Source James Kay , Keith Baker , Nigel Hammond , William Hoffman , A. Thomson MEDSI 2008 Show Abstract ▼ Abstract: Floor stability spoecification; civil engineering, design and construction; description of the HLS system;	Jun 2008

Publications Database

Search Results

You searched for all publications:

Search Again...

Subject Areas (check all that apply) select all

Instruments used to collect data (check all that apply) select all

Collaborations involved (check all that apply) select all

Diamond Offline Facilities used

Relevant Technical Areas (check all that apply) select all

Menu for Anonymous User