Search Results

You searched for all publications:

with publication states 'Published' 'Published (Approved)'
with Tags 'Biomaterials'

Search Again...

These results include publications that have not yet been reviewed and processed by Diamond Light Source. To view only processed publications click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

Exact matches
Related results

82 items found (0 items not approved), displaying 31 to 40.[First/Prev] 1, 2, 3, 4, 5, 6, 7, 8 [Next/Last]

Instruments / Technical Area	Publication Details	Published
B21-High Throughput SAXS	Rapid and direct preparation of lignin nanoparticles from alkaline pulping liquor by mild ultrasonication Melissa B. Agustin , Paavo A. Penttilä , Maarit Lahtinen , Kirsi S. Mikkonen Acs Sustainable Chemistry & Engineering DOI: 10.1021/acssuschemeng.9b05445 Diamond Proposal Number(s): [24748] Show Abstract ▼ Abstract: The production of lignin nanoparticles (LNPs) has opened new routes to utilization of lignin in advanced applications. The existing challenge, however, is to develop a production method that can easily be adapted on an industrial scale. In this study, we demonstrated a green and rapid method of preparing LNPs directly from a sulfur-free alkaline pulping liquor by combining acid-precipitation and ultrasonication. The combined method produced spherical LNPs, with hierarchical nanostructure and highly negative surface charge, within only 5-min of sonication. The mild, rapid sonication was achieved by sonicating directly without prior drying the acid-precipitated and dialyzed lignin. Optimization of the method revealed the potential for minimizingacid consumption, shortening the dialysis time, and processing directly the alkaline liquor with as much as 20 wt% lignin. The isolated LNPs were stable during storage for 180 days, at a pH range of 4–7 and in a dispersing medium below 0.1 M NaCl. The LNPs also displayed excellent emulsifying properties, stabilizing oil-in-water emulsions. Thus, this simple and energy-efficient method opens a sustainable, straightforward and scalable route to production of solvent-free LNPs, with high potential as interface stabilizers of multi-phase systems in the food and medical industries.	Nov 2019
I04-1-Macromolecular Crystallography (fixed wavelength)	Biomacromolecular charge chirality detected using chiral plasmonic nanostructures Marion Rodier , Chantal Keijzer , Joel Milner , Affar S. Karimullah , Aleksander W. Roszak , Laurence D. Barron , Nikolaj Gadegaard , Adrian J. Lapthorn , Malcolm Kadodwala Nanoscale Horizons 45 DOI: 10.1039/C9NH00525K Diamond Proposal Number(s): [16258, 16258] Open Access Show Abstract ▼ Abstract: The charge distributions of solvent exposed surfaces of complex biomolecules such has proteins are unique fingerprints. The chirality of these charge distributions result in stereo-specific electrostatic interactions which help define how proteins interact with each other, contributing to specificity in protein–protein interactions. Thus it is a key concept in understanding chemical processes in biology. There is currently no known spectroscopic phenomenon that allows rapid characterisation of chiral surface charge distributions. We show that this essential property that is currently “invisible” to optical spectroscopy, can be detected by monitoring asymmetries in the chiroptical response of protein–plasmonic nanostructure complexes. The unique capabilities of the phenomenon are utilised to discriminate between a structurally homologous series of proteins, type II dehydroquinase (DHQase) derived from different organisms. The proteins are indistinguishable with conventional structurally sensitive spectroscopy (i.e. circular dichroism). We show that discrimination between proteins can be achieved by detecting differences in chiral surface charge distributions. The phenomenon is explained with a simple model whereby the chiroptical properties of the plasmonic structures are perturbed by the induction of an enantiomeric mirror image charge distribution of the protein in the metal. This new phenomenon has broad impact, it is a powerful tool for discriminating between structurally homologous biomaterials, but will also provide information relevant to macromolecular interactions.	Oct 2019
I22-Small angle scattering & Diffraction	Matrix-induced pre-strain and mineralization-dependent interfibrillar shear transfer enable 3D fibrillar deformation in a biogenic armour Yanhong Wang , Yi Zhang , Nicholas J. Terrill , Ettore Barbieri , Nicola M. Pugno , Himadri Gupta Acta Biomaterialia DOI: 10.1016/j.actbio.2019.09.036 Diamond Proposal Number(s): [17869] Show Abstract ▼ Abstract: The cuticle of stomatopod is an example of a natural mineralized biomaterial, consisting of chitin, amorphous calcium carbonate and protein components with a multiscale hierarchical structure, and forms a protective shell with high impact resistance. At the ultrastructural level, cuticle mechanical functionality is enabled by the nanoscale architecture, wherein chitin fibrils are in intimate association with enveloping mineral and proteins. However, the interactions between these ultrastructural building blocks, and their coupled response to applied load, remain unclear. Here, we elucidate these interactions via synchrotron microbeam wide-angle X-ray diffraction combined with in situ tensile loading, to quantify the chitin crystallite structure of native cuticle – and after demineralization and deproteinization – as well as time-resolved changes in chitin fibril strain on macroscopic loading. We demonstrate chitin crystallite stabilization by mineral, seen via a compressive pre-strain of approximately 0.10% (chitin/protein fibre pre-stress of ∼20 MPa), which is lost on demineralization. Clear reductions of stiffness at the fibrillar-level following matrix digestion are linked to the change in the protein/matrix mechanical properties. Furthermore, both demineralization and deproteinization alter the 3D-pattern of deformation of the fibrillar network, with a non-symmetrical angular fibril strain induced by the chemical modifications, associated with loss of the load-transferring interfibrillar matrix. Our results demonstrate and quantify the critical role of interactions at the nanoscale (between chitin-protein and chitin-mineral) in enabling the molecular conformation and outstanding mechanical properties of cuticle, which will inform future design of hierarchical bioinspired composites.	Sep 2019
I03-Macromolecular Crystallography	The ZT biopolymer: A self-assembling protein scaffold for stem cell applications Yevheniia Nesterenko , Christopher J. Hill , Jennifer R. Fleming , Patricia Murray , Olga Mayans International Journal Of Molecular Sciences 20 DOI: 10.3390/ijms20174299 Open Access Show Abstract ▼ Abstract: The development of cell culture systems for the naturalistic propagation, self-renewal and differentiation of cells ex vivo is a high goal of molecular engineering. Despite significant success in recent years, the high cost of up-scaling cultures, the need for xeno-free culture conditions, and the degree of mimicry of the natural extracellular matrix attainable in vitro using designer substrates continue to pose obstacles to the translation of cell-based technologies. In this regard, the ZT biopolymer is a protein-based, stable, scalable, and economical cell substrate of high promise. ZT is based on the naturally occurring assembly of two human proteins: titin-Z1Z2 and telethonin. These protein building blocks are robust scaffolds that can be conveniently functionalized with full-length proteins and bioactive peptidic motifs by genetic manipulation, prior to self-assembly. The polymer is, thereby, fully encodable. Functionalized versions of the ZT polymer have been shown to successfully sustain the long-term culturing of human embryonic stem cells (hESCs), human induced pluripotent stem cells (hiPSCs), and murine mesenchymal stromal cells (mMSCs). Pluripotency of hESCs and hiPSCs was retained for the longest period assayed (4 months). Results point to the large potential of the ZT system for the creation of a modular, pluri-functional biomaterial for cell-based applications.	Sep 2019
B23-Circular Dichroism I22-Small angle scattering & Diffraction	Thermally robust solvent-free biofluids of M13 bacteriophage engineered for high compatibility with anhydrous ionic liquids Alex P. S. Brogan , Nimrod Heldman , Jason P. Hallett , Angela M. Belcher Chemical Communications 540 DOI: 10.1039/C9CC04909F Diamond Proposal Number(s): [16396] Show Abstract ▼ Abstract: Here, we demonstrate a chemical modification strategy to create biomaterials of the M13 bacteriophage with extraordinary thermal stability, and high compatibility with non-aqueous ionic liquids. The results provide a blueprint for developing soft materials with well-defined architectures that may find broad applicability in the next generation of flexible devices.	Aug 2019
I13-2-Diamond Manchester Imaging	Rapid fabrication of reinforced and cell-laden vascular grafts structurally inspired by human coronary arteries Tamara L. Akentjew , Claudia Terraza , Cristian Suazo , Jekaterina Maksimuck , Camila A. Wilkens , Francisco Vargas , Gabriela Zavala , Macarena Ocaña , Javier Enrione , Claudio M. García-Herrera , Loreto M. Valenzuela , Jonny J. Blaker , Maroun Khoury , Juan Pablo Acevedo Nature Communications 10 DOI: 10.1038/s41467-019-11090-3 Diamond Proposal Number(s): [15507] Open Access Show Abstract ▼ Abstract: Design strategies for small diameter vascular grafts are converging toward native-inspired tissue engineered grafts. A new automated technology is presented that combines a dip-spinning methodology for depositioning concentric cell-laden hydrogel layers, with an adapted solution blow spinning (SBS) device for intercalated placement of aligned reinforcement nanofibres. This additive manufacture approach allows the assembly of bio-inspired structural configurations of concentric cell patterns with fibres at specific angles and wavy arrangements. The middle and outer layers were tuned to structurally mimic the media and adventitia layers of native arteries, enabling the fabrication of small bore grafts that exhibit the J-shape mechanical response and compliance of human coronary arteries. This scalable automated system can fabricate cellularized multilayer grafts within 30 min. Grafts were evaluated by hemocompatibility studies and a preliminary in vivo carotid rabbit model. The dip-spinning-SBS technology generates constructs with native mechanical properties and cell-derived biological activities, critical for clinical bypass applications.	Jul 2019
I13-2-Diamond Manchester Imaging	Sintering and concomitant crystallization of bioactive glasses Carsten Blaeß , Ralf Müller , Gowsihan Poologasundarampillai , Delia S. Brauer International Journal Of Applied Glass Science 5 DOI: 10.1111/ijag.13477 Diamond Proposal Number(s): [17001] Open Access Show Abstract ▼ Abstract: The sintering of bioactive glasses allows for the preparation of complex structures, such as three‐dimensional porous scaffolds. Such 3D constructs are particularly interesting for clinical applications of bioactive glasses in bone regeneration, as the scaffolds can act as a guide for in‐growing bone cells, allowing for good integration with existing and newly formed tissue while the scaffold slowly degrades. Owing to the pronounced tendency of many bioactive glasses to crystallize upon heat treatment, 3D scaffolds have not been much exploited commercially. Here, we investigate the influence of crystallization on the sintering behavior of several bioactive glasses. In a series of mixed‐alkali glasses an increased CaO/alkali metal oxide ratio improved sintering compared to Bioglass 45S5, where dense sintering was inhibited. Addition of small amounts of calcium fluoride helped to keep melting and sintering temperatures low. Unlike glass 13‐93, these new glasses crystallized during sintering but this did not prevent densification. Variation in bioactive glass particle size allowed for fine‐tuning the microporosity resulting from the sintering process.	Jul 2019
I22-Small angle scattering & Diffraction	Multifunctional nano‐biointerfaces: cytocompatible antimicrobial nanocarriers from stabilizer‐free cubosomes Mahsa Zabara , Berna Senturk , Mark Gontsarik , Qun Ren , Markus Rottmar , Katharina Maniura‐weber , Raffaele Mezzenga , Sreenath Bolisetty , Stefan Salentinig Advanced Functional Materials 2 DOI: 10.1002/adfm.201904007 Show Abstract ▼ Abstract: The rational design of alternative antimicrobial materials with reduced toxicity toward mammalian cells is highly desired due to the growing occurrence of bacteria resistant to conventional antibiotics. A promising approach is the design of lipid‐based antimicrobial nanocarriers. However, most of the commonly used polymer‐stabilized nanocarriers are cytotoxic. Herein, the design of a novel, stabilizer‐free nanocarrier for the human cathelicidin derived antimicrobial peptide LL‐37 that is cytocompatible and promotes cell proliferation for improved wound healing is reported. The nanocarrier is formed through the spontaneous integration of LL‐37 into novel, stabilizer‐free glycerol mono‐oleate (GMO)‐based cubosomes. Transformations in the internal structure of the cubosomes from Pn3m to Im3m‐type and eventually their transition into small vesicles and spherical micelles are demonstrated upon the encapsulation of LL‐37 into their internal bicontinuous cubic structure using small angle X‐ray scattering, cryogenic transmission electron microscopy, and light scattering techniques. Additional in vitro biological assays show the antimicrobial activity of the stabilizer‐free nano‐objects on a variety of bacteria strains, their cytocompatibility, and cell‐proliferation enhancing effect. The results outline a promising strategy for the comprehensive design of antimicrobial, cytocompatible lipid nanocarriers for the protection and delivery of bioactive molecules with potential for application as advanced wound healing materials.	Jul 2019
I16-Materials and Magnetism I22-Small angle scattering & Diffraction	Structural evidence that the polymerization rate dictates order and intrinsic strain generation in photocured methacrylate biomedical polymers Slobodan Sirovica , Maximilian W. A. Skoda , Maciej Podgorski , Paul B. J. Thompson , William M. Palin , Yilan Guo , Andrew J. Smith , Karun Dewan , Owen Addison , Richard Martin Macromolecules DOI: 10.1021/acs.macromol.9b00133 Diamond Proposal Number(s): [11687, 14117, 15319] Open Access Show Abstract ▼ Abstract: The influence of reaction rate on the evolving polymer structure of photo-activated dimethacrylate biomedical resins was investigated using neutron and in situ synchrotron X-ray scattering with simultaneous Fourier-transform-near-infrared spectroscopy. Previous studies have correlated the degree of reactive group conversion with mechanical properties, but the impact of polymerization rate on the resultant polymer structure is unknown. Here, we demonstrate that the medium-range structural order at the functional end groups of these materials is dependent on the reaction rate. Accelerating polymerization increases correlation lengths in the methacrylate end groups but reduces the medium-range structural order per converted vinyl bond when compared with more slowly polymerized systems. At faster rates of polymerization, the conformation of atoms at the reacting end group can become fixed into the polymer structure at the onset of autodeceleration, storing residual strain. Neutron scattering confirms that the structural differences observed are reproduced at longer length scales. This effect is not as prominent in systems polymerized at slower rates despite similar final degrees of reactive group conversion. Results suggest that current interpretations of these materials, which extrapolate mechanical properties from conversion, may be incomplete. Accelerating polymerization can introduce structural differences, which will dictate residual strain and may ultimately explain the discrepancies in the predictive modeling of the mechanical behavior of these materials using conventional techniques.	Jul 2019
I07-Surface & interface diffraction	Interaction of a tripeptide with titania surfaces: RGD adsorption on rutile TiO2(110) and model dental implant surfaces Michael Wagstaffe , Hadeel Hussain , Mark Taylor , Matthew Murphy , Nikolaos Silikas , Andrew G. Thomas Materials Science And Engineering: C DOI: 10.1016/j.msec.2019.110030 Show Abstract ▼ Abstract: The adsorption of peptides on metal oxides is an area of significant interest, both fundamentally and in a number of technologically important areas. These range from the integration of biomaterials in the body, to denaturation of protein therapeutics and the use of biomolecules and bioinspired materials in synthesis and stabilization of novel nanomaterials. Here we present a study of the tripeptide arginylglycylaspartic acid (RGD) on the surfaces of vacuum-prepared single crystalline TiO2(110), pyrocatechol-capped TiO2(110), and model SLA and SLActive dental implant samples. X-ray Photoelectron Spectroscopy and Scanning Tunneling Microscopy show that the RGD adsorption mode on the single crystal is consistent with bonding through the deprotonated carboxylate groups of the peptide to surface Ti atoms of the substrate. Despite the increased hydrophobicity of the pyrocatechol-capped TiO2(110) surface RGD adsorption from solution increases following this surface treatment. RGD adsorption on SLA and SLActive surfaces shows that the SLActive surface has a greater uptake of RGD. The RGD uptake on the pyrocatechol capped single crystal and the model implant surfaces suggest that the ease with which surface contaminant hydrocarbons are removed from the surface has a greater influence on peptide adsorption than hydrophobicity/hydrophilicity of the surface.	Jul 2019

Publications Database

Search Results

You searched for all publications:

Search Again...

Subject Areas (check all that apply) select all

Instruments used to collect data (check all that apply) select all

Collaborations involved (check all that apply) select all

Diamond Offline Facilities used

Relevant Technical Areas (check all that apply) select all

Menu for Anonymous User