Diamond Publications - Search Results

Search Again...

These results include publications that have not yet been reviewed and processed by Diamond Light Source. To view only processed publications click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
I13-1-Coherence I18-Microfocus Spectroscopy	Developing a system for in vivo imaging of maize roots containing iodinated contrast media in soil using synchrotron XCT and XRF Callum P. Scotson , Arjen Van Veelen , Katherine A. Williams , Nicolai Koebernick , Dan Mckay Fletcher , Tiina Roose Plant And Soil 4 DOI: 10.1007/s11104-020-04784-x Diamond Proposal Number(s): [18762, 19399] Open Access Show Abstract ▼ Abstract: Aims: We sought to develop a novel experimental system which enabled application of iodinated contrast media to in vivo plant roots intact in soil and was compatible with time-resolved synchrotron X-ray computed tomography imaging. The system was developed to overcome issues of low contrast to noise within X-ray computed tomography images of plant roots and soil environments, the latter of which can complicate image processing and result in the loss of anatomical information. Methods: To demonstrate the efficacy of the system we employ the novel use of both synchrotron X-ray computed tomography and synchrotron X-ray fluorescence mapping to capture the translocation of the contrast media through root vasculature into the leaves. Results: With the application of contrast media we identify fluid flow in root vasculature and visualise anatomical features, which are otherwise often only observable in ex vivo microscopy, including: the xylem, metaxylem, pith, fibres in aerenchyma and leaf venation. We are also able to observe interactions between aerenchyma cross sectional area and solute transport in the root vasculature with depth. Conclusions: Our novel system was capable of successfully delivering sufficient contrast media into root and leaf tissues such that anatomical features could be visualised and internal fluid transport observed. We propose that our system could be used in future to study internal plant transport mechanisms and parameterise models for fluid flow in plants.	Dec 2020

Instruments / Technical Area

Publication Details

Published

Actions

I13-1-Coherence
I18-Microfocus Spectroscopy

Developing a system for in vivo imaging of maize roots containing iodinated contrast media in soil using synchrotron XCT and XRF

Callum P. Scotson , Arjen Van Veelen , Katherine A. Williams , Nicolai Koebernick , Dan Mckay Fletcher , Tiina Roose

Plant And Soil 4

DOI: 10.1007/s11104-020-04784-x

Diamond Proposal Number(s): [18762, 19399]