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Instruments / Technical Area	Publication Details	Published	Actions
B21-High Throughput SAXS I03-Macromolecular Crystallography	Non-canonical Staphylococcus aureus pathogenicity island repression Laura Miguel-Romero , Mohammed Alqasmi , Julio Bacarizo , Jason A. Tan , Richard J. Cogdell , John Chen , Olwyn Byron , Gail E. Christie , Alberto Marina , José R. Penadés Nucleic Acids Research 2 DOI: 10.1093/nar/gkac855 Diamond Proposal Number(s): [16258] Open Access Show Abstract ▼ Abstract: Mobile genetic elements control their life cycles by the expression of a master repressor, whose function must be disabled to allow the spread of these elements in nature. Here, we describe an unprecedented repression-derepression mechanism involved in the transfer of Staphylococcus aureus pathogenicity islands (SaPIs). Contrary to the classical phage and SaPI repressors, which are dimers, the SaPI1 repressor StlSaPI1 presents a unique tetrameric conformation never seen before. Importantly, not just one but two tetramers are required for SaPI1 repression, which increases the novelty of the system. To derepress SaPI1, the phage-encoded protein Sri binds to and induces a conformational change in the DNA binding domains of StlSaPI1, preventing the binding of the repressor to its cognate StlSaPI1 sites. Finally, our findings demonstrate that this system is not exclusive to SaPI1 but widespread in nature. Overall, our results characterize a novel repression-induction system involved in the transfer of MGE-encoded virulence factors in nature.	Oct 2022

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B21-High Throughput SAXS
I03-Macromolecular Crystallography

Non-canonical Staphylococcus aureus pathogenicity island repression

Laura Miguel-Romero , Mohammed Alqasmi , Julio Bacarizo , Jason A. Tan , Richard J. Cogdell , John Chen , Olwyn Byron , Gail E. Christie , Alberto Marina , José R. Penadés

Nucleic Acids Research 2

DOI: 10.1093/nar/gkac855

Diamond Proposal Number(s): [16258]