Search Results

You searched for all publications:

with publication states 'Published' 'Published (Approved)'

Search Again...

These results include publications that have not yet been reviewed and processed by Diamond Light Source. To view only processed publications click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

Exact matches
Related results

128 items found (1 item not approved), displaying 1 to 10.[First/Prev] 1, 2, 3, 4, 5, 6, 7, 8 [Next/Last]

Instruments / Technical Area	Publication Details	Published	Actions
	Applications of Circular Dichroism Giuliano Siligardi , Rohanah Hussain DOI: 10.1016/B978-0-12-374413-5.00031-2 Show Abstract ▼ Abstract: Circular dichroism (CD) spectroscopy is the technique of choice to study chiral molecules in solution, in particular biologically important molecules such as proteins, nucleic acids, carbohydrates, and therapeutic drugs. An important application of CD spectroscopy is the determination of the equilibrium dissociation constant, Kd, of binding interactions between a macromolecular host and a ligand. The Kd can be calculated by analyzing CD data at fixed wavelength versus ligand concentration using a nonlinear regression method. Several examples of CD titrations are illustrated here to describe the ‘golden’ rules on how to determine the molecular binding interactions of nonimmobilized and nonlabeled systems of biologically important molecules by CD spectroscopy.	Jan 2010
	Length-dependent electron spin polarization in oligopeptides and DNA Suryakant Mishra , Amit Kumar Mondal , Shubhadeep Pal , Tapan Kumar Das , Eilam Z. B. Smolinsky , Giuliano Siligardi , Ron Naaman The Journal Of Physical Chemistry C DOI: 10.1021/acs.jpcc.0c02291 Show Abstract ▼ Abstract: The effect of spin polarization in conduction and electric field-induced polarization was measured for double-stranded DNA oligonucleotides and oligopeptides of different lengths. The measurements were conducted using magnetic contact AFM, spin-dependent electrochemistry, spin-dependent polarization, and magnetoresistance studies. It was established that the spin-dependent conduction through chiral molecules depends on the voltage applied with a power of d, when d is larger than unity, and that there is a different voltage threshold for conducting each of the spin polarizations. In addition, there is no spin flipping during the conduction through the chiral system. The spin polarization depends linearly on the length, within the range of lengths studied, and it seems to scale like the optical activity. These results suggest the importance of the electric polarizability in the chiral-induced spin selectivity process.	Apr 2020
	CDApps: integrated software for experimental planning and data processing at beamline B23, Diamond Light Source. Corrigendum Rohanah Hussain , Kristian Benning , Daniel Myatt , Tamas Javorfi , Edoardo Longo , Timothy R. Rudd , Bill Pulford , Giuliano Siligardi Journal Of Synchrotron Radiation 22 (3), 862 - 862 DOI: 10.1107/S1600577515007602 PMID: 25931108 Open Access Show Abstract ▼ Abstract: In the paper by Hussain et al. [(2015), J. Synchrotron Rad. 22, 465-468 ], Daniel Myatt is missing from the list of authors. The complete list of authors should be Rohanah Hussain, Kristian Benning, Daniel Myatt, Tamas Javorfi, Edoardo Longo, Timothy R. Rudd, Bill Pulford and Giuliano Siligardi.	May 2015
	Ligand interactions: Challenges ahead using SRCD Rohanah Hussain , Guiliano Siligardi 2nd International Workshop on Synchrotron Radiation Circular Dichroism Spectroscopy	Apr 2009
	A novel drug delivery approach for the formulation of lipophilic therapeutics of small molecule peptide-based and protein drugs Rohanah Hussain , Giuliano Siligardi 12th International Conference on Circular Dichroism	Aug 2009
	A novel cationic lipopeptide therapeutics with a broad range of antimicrobial activites Rohanah Hussain , Giuliano Siligardi 12th International Conference on Circular Dichroism	Aug 2009
	The investigation of an anti-cancer drug (Flavopiridol) binding to blood serum albumin Daniel Myatt , Sonja Baumli , Guiliano Siligardi , Louise Johnson 12th International Conference on Circular Dichroism	Aug 2009
	Effect of 4-Fluoro-L-proline on the SH3 Binding Affinity Paolo Ruzza , Chiara Rubini , Andrea Calderan , Andrea Guiotto , Luca Cesaro , Anna M. Brunati , Arianna Donella-deana , Rohanah Hussain , Giuliano Siligardi Peptides For Youth: The Proceedings Of The 20th American Peptide Symposium 611, 499-500 DOI: 10.1007/978-0-387-73657-0_216 PMID: 19400283	Mar 2009
	4-Fluoroproline derivative peptides: effect on PPII conformation and SH3 affinity Paolo Ruzza , Giuliano Siligardi , Arianna Donella-deana , Andrea Calderan , Rohanah Hussain , Chiara Rubini , Luca Cesaro , Alessio Osler , Andrea Guiotto , Lorenzo A. Pinna , Gianfranco Borin Journal Of Peptide Science 12 (7), 462-471 DOI: 10.1002/psc.750 PMID: 16506148 Show Abstract ▼ Abstract: Eukaryotic signal transduction involves the assembly of transient protein-protein complexes mediated by modular interaction domains. Specific Pro-rich sequences with the consensus core motif PxxP adopt the PPII helix conformation upon binding to SH3 domains. For short Pro-rich peptides, little or no ordered secondary structure is usually observed before binding interactions. The association of a Pro-rich peptide with the SH3 domain involves unfavorable binding entropy due to the loss of rotational freedom on forming the PPII helix. With the aim of stabilizing the PPII helix conformation in the Pro-rich HPKI decapeptide PPPLPPKPKF (P2), a series of P2 analogues was prepared, in which specific Pro positions were alternatively occupied by 4(S)- or 4(R)-4-fluoro-L-proline. The interactions of these peptides with the SH3 domain of the HPK1-binding partner HS1 were quantitatively analyzed by the NILIA-CD approach. A CD thermal analysis of the P2 analogues was performed to assess their propensity to adopt the PPII helix conformation. Contrary to our expectations, the K-d values of the analogues were lower than that of the parent peptide P2. These results clearly show that the induction of a stable PPII helix conformation in short Pro-rich peptides is not sufficient to increase their affinity toward the SH3 domain and that the effect of 4-fluoroproline strongly depends on the position of this residue in the sequence and the chirality of the substituent in the pyrrolidine ring. Copyright (c) 2006 European Peptide Society and John Wiley & Sons, Ltd.	Jul 2006
	Fat-free Albumin as a Novel Drug Delivery System Rohanah Hussain , Giuliano Siligardi International Journal Of Peptide Research And Therapeutics 12 (3), 311-315 DOI: 10.1007/s10989-006-9028-1 Show Abstract ▼ Abstract: The search for effective drug delivery systems is one of the major challenges in drug formulation especially for biopharmaceuticals Such as proteins, and peptide-based drugs and vaccines. A procedure has been developed whereby human serum albumin (HSA) call be Used as a delivery vehicle for these biomolecules using its role as main ratty acid carrier. Using essentially fatty acid free HSA (HSAff) it is possible to form stable complexes with lipidic chain compounds (lipo-compounds). Two lipo-compounds have been used to develop this system, a novel antimicrobial lipopeptide and gamma-amino-n-butyric acid, GABA. conjugated with an alkyl chain, lipo-GABA, in both cases C8 and C14 alkyl chain lengths were evaluated. The HAS-lipo compound complex had a mutual stabilizing effect on both the HSA and the lipo-compound. The protease enzyme study showed that the alkyl chains of these lipo-compounds bound to HSAff confer a similar if not greater biostability than caprylic acid shown by CD and importantly, the bound lipopeptide was stabilized by the HSA shown by mass spectrometry. Heat stability studies at 60 degrees C over 10 h also confirmed that the lipo-HSA complexes confer stability and provide a method of preparing sterile formulation for therapeutic use. No further increased in stability of the lipo-compounds when HSA containing fatty acid (HSAfa) Was used. With the antimicrobial lipopeptide, there was enhanced activity with HSAff formulation suggesting increased biostability and bioavailability of compounds. These finding allowed us to develop a simple and effective way of delivering lipo-compounds using fatty acid free HSA as the carrier.	Sep 2006

Publications Database

Search Results

You searched for all publications:

Search Again...

Subject Areas (check all that apply) select all

Instruments used to collect data (check all that apply) select all

Collaborations involved (check all that apply) select all

Diamond Offline Facilities used

Relevant Technical Areas (check all that apply) select all

Menu for Anonymous User