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Instruments / Technical Area	Publication Details	Published	Actions
	The HARE chip for efficient time-resolved serial synchrotron crystallography Pedram Mehrabi , Henrike Mueller-Werkmeister , Jan-Philipp Leimkohl , Hendrik Schikora , Jelena Ninkovic , Silvia Krivokuca , Ladislav Andriček , Sascha W. Epp , Darren A. Sherrell , Robin L. Owen , Arwen R. Pearson , Friedjof Tellkamp , Eike Schulz , R. J. Dwayne Miller Journal Of Synchrotron Radiation 27, 360 - 370 DOI: 10.1107/S1600577520000685 Open Access Show Abstract ▼ Abstract: Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided.	Mar 2020
I24-Microfocus Macromolecular Crystallography	Low-dose fixed-target serial synchrotron crystallography Robin L. Owen , Danny Axford , Darren A. Sherrell , Anling Kuo , Oliver P. Ernst , Eike Schulz , R. J. Dwayne Miller , Henrike Mueller-Werkmeister Acta Crystallographica Section D Structural Biology 73, 373 - 378 DOI: 10.1107/S2059798317002996 Diamond Proposal Number(s): [11175] Open Access Show Abstract ▼ Abstract: The development of serial crystallography has been driven by the sample requirements imposed by X-ray free-electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed-target approach to high-throughput serial sampling, it is demonstrated that high-quality data can be collected from myoglobin crystals, allowing room-temperature, low-dose structure determination. The combination of fixed-target arrays and a fast, accurate translation system allows high-throughput serial data collection at high hit rates and with low sample consumption.	Apr 2017

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