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Instruments / Technical Area	Publication Details	Published	Actions
B21-High Throughput SAXS	Merging in-solution x-ray and neutron scattering data allows fine structural analysis of membrane–protein detergent complexes Gaetan Dias Mirandela , Giulia Tamburrino , Miloš T. Ivanović , Felix M. Strnad , Olwyn Byron , Tim Rasmussen , Paul A. Hoskisson , Jochen S. Hub , Ulrich Zachariae , Frank Gabel , Arnaud Javelle The Journal Of Physical Chemistry Letters DOI: 10.1021/acs.jpclett.8b01598 Diamond Proposal Number(s): [15616] Open Access Show Abstract ▼ Abstract: In-solution small-angle X-ray and neutron scattering (SAXS/SANS) have become popular methods to characterize the structure of membrane proteins, solubilized by either detergents or nanodiscs. SANS studies of protein-detergent complexes usually require deuterium-labeled proteins or detergents, which in turn often lead to problems in their expression or purification. Here, we report an approach whose novelty is the combined analysis of SAXS and SANS data from an unlabeled membrane protein complex in solution in two complementary ways. First, an explicit atomic analysis, including both protein and detergent molecules, using the program WAXSiS, which has been adapted to predict SANS data. Second, the use of MONSA which allows one to discriminate between detergent head- and tail-groups in an ab initio approach. Our approach is readily applicable to any detergent-solubilized protein and provides more detailed structural information on protein–detergent complexes from unlabeled samples than SAXS or SANS alone.	Jul 2018

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B21-High Throughput SAXS

Merging in-solution x-ray and neutron scattering data allows fine structural analysis of membrane–protein detergent complexes