Diamond Publications - Search Results

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
B21-High Throughput SAXS	Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation Paula M. Corsini , Sunjun Wang , Saima Rehman , Katherine Fenn , Amin Sagar , Slobodan Sirovica , Leanne Cleaver , Charlotte J. C. Edwards-Gayle , Giulia Mastroianni , Ben Dorgan , Lee M. Sewell , Steven Lynham , Dinu Iuga , W. Trent Franks , James Jarvis , Guy H. Carpenter , Michael. A. Curtis , Pau Bernadó , Vidya C. Darbari , James A. Garnett Npj Biofilms And Microbiomes 8 DOI: 10.1038/s41522-022-00272-5 Diamond Proposal Number(s): [24690] Open Access Show Abstract ▼ Abstract: Escherichia coli is a Gram-negative bacterium that colonises the human intestine and virulent strains can cause severe diarrhoeal and extraintestinal diseases. The protein SslE is secreted by a range of pathogenic and commensal E. coli strains. It can degrade mucins in the intestine, promotes biofilm maturation and it is a major determinant of infection in virulent strains, although how it carries out these functions is not well understood. Here, we examine SslE from the commensal E. coli Waksman and BL21 (DE3) strains and the enterotoxigenic H10407 and enteropathogenic E2348/69 strains. We reveal that SslE has a unique and dynamic structure in solution and in response to acidification within mature biofilms it can form a unique aggregate with amyloid-like properties. Furthermore, we show that both SslE monomers and aggregates bind DNA in vitro and co-localise with extracellular DNA (eDNA) in mature biofilms, and SslE aggregates may also associate with cellulose under certain conditions. Our results suggest that interactions between SslE and eDNA are important for biofilm maturation in many E. coli strains and SslE may also be a factor that drives biofilm formation in other SslE-secreting bacteria.	Feb 2022

Instruments / Technical Area

Publication Details

Published

Actions

B21-High Throughput SAXS

Molecular and cellular insight into Escherichia coli SslE and its role during biofilm maturation