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Instruments / Technical Area	Publication Details	Published	Actions
B22-Multimode InfraRed imaging And Microspectroscopy	Hyperspectral mapping of human primary and stem cells at cell-matrix interfaces Emiliana De Santis , Nilofar Faruqui , Craig T. Russell , James E. Noble , Ibolya E. Kepiro , Katharine Hammond , Maria Tsalenchuk , Eugeni M. Ryadnov , Magda Wolna , Mark D. Frogley , Christopher J. Price , Ivana Barbaric , Gianfelice Cinque , Maxim G. Ryadnov Acs Applied Materials & Interfaces DOI: 10.1021/acsami.3c17113 Diamond Proposal Number(s): [16548] Show Abstract ▼ Abstract: Extracellular matrices interface with cells to promote cell growth and tissue development. Given this critical role, matrix mimetics are introduced to enable biomedical materials ranging from tissue engineering scaffolds and tumor models to organoids for drug screening and implant surface coatings. Traditional microscopy methods are used to evaluate such materials in their ability to support exploitable cell responses, which are expressed in changes in cell proliferation rates and morphology. However, the physical imaging methods do not capture the chemistry of cells at cell–matrix interfaces. Herein, we report hyperspectral imaging to map the chemistry of human primary and embryonic stem cells grown on matrix materials, both native and artificial. We provide the statistical analysis of changes in lipid and protein content of the cells obtained from infrared spectral maps to conclude matrix morphologies as a major determinant of biochemical cell responses. The study demonstrates an effective methodology for evaluating bespoke matrix materials directly at cell–matrix interfaces.	Jan 2024

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B22-Multimode InfraRed imaging And Microspectroscopy

Hyperspectral mapping of human primary and stem cells at cell-matrix interfaces