Diamond Publications - Search Results

Search Again...

These results include publications that have not yet been reviewed and processed by Diamond Light Source. To view only processed publications click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
I03-Macromolecular Crystallography	Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism Fiona Cuskin , Elisabeth C. Lowe , Max J. Temple , Yanping Zhu , Elizabeth A. Cameron , Nicholas A. Pudlo , Nathan T. Porter , Karthik Urs , Andrew J. Thompson , Alan Cartmell , Artur Rogowski , Brian S. Hamilton , Rui Chen , Thomas J. Tolbert , Kathleen Piens , Debby Bracke , Wouter Vervecken , Zalihe Hakki , Gaetano Speciale , Jose L. Munoz-Munoz Nature 517 (7533), 165 - 169 DOI: 10.1038/nature13995 Diamond Proposal Number(s): [7864] Show Abstract ▼ Abstract: Yeasts, which have been a component of the human diet for at least 7,000 years, possess an elaborate cell wall α-mannan. The influence of yeast mannan on the ecology of the human microbiota is unknown. Here we show that yeast α-mannan is a viable food source for the Gram-negative bacterium Bacteroides thetaiotaomicron, a dominant member of the microbiota. Detailed biochemical analysis and targeted gene disruption studies support a model whereby limited cleavage of α-mannan on the surface generates large oligosaccharides that are subsequently depolymerized to mannose by the action of periplasmic enzymes. Co-culturing studies showed that metabolism of yeast mannan by B. thetaiotaomicron presents a selfish model for the catabolism of this difficult to breakdown polysaccharide. Genomic comparison with B. thetaiotaomicron in conjunction with cell culture studies show that a cohort of highly successful members of the microbiota has evolved to consume sterically-restricted yeast glycans, an adaptation that may reflect the incorporation of eukaryotic microorganisms into the human diet.	Jan 2015

Instruments / Technical Area

Publication Details

Published

Actions

I03-Macromolecular Crystallography

Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism