Diamond Publications - Search Results

Search Again...

These results only include publications that have been reviewed and processed by Diamond Light Source. To also view papers that have not yet been processed click here.

You can use the folder icon under Actions to collate papers as required. You can then click on View Folder in the left-hand navigation to review and/or download your folder.

One item found (0 items not approved).

Instruments / Technical Area	Publication Details	Published	Actions
B21-High Throughput SAXS	Coarse-grained modeling of antibodies from small-angle scattering profiles Daniel Corbett , Max Hebditch , Rose Keeling , Peng Ke , Sofia Ekizoglou , Prasad Sarangapani , Jai Pathak , Christopher F. Van Der Walle , Shahid Uddin , Clair Baldock , Carlos Avendaño , Robin A. Curtis The Journal Of Physical Chemistry B 121, 8276 - 8290 DOI: 10.1021/acs.jpcb.7b04621 Diamond Proposal Number(s): [10510] Show Abstract ▼ Abstract: Predicting the concentrated solution behavior for monoclonal antibodies requires developing and using minimal models to describe their shape and interaction potential. Toward this end, the small-angle X-ray scattering (SAXS) profiles for a monoclonal antibody (COE-03) have been measured under solution conditions chosen to produce weak self-association. The experiments are complemented with molecular simulations of a three-bead antibody model with and without interbead attraction. The scattering profile is extracted directly from the molecular simulation to avoid using the decoupling approximation. We examine the ability of the three-bead model to capture features of the scattering profile and the dependence of compressibilty on protein concentration. The three-bead model is able to reproduce generic features of the experimental structure factor as a function of wave vector S(k) including a well-defined shoulder, which is a consequence of the planar structure of the antibody, and a well-defined minimum in S(k) at k ∼ 0.025 Å–1. We also show the decoupling approximation is incapable of accounting for highly anisotropic shapes. The best-fit parameters obtained from matching spherical models to simulated scattering profiles are protein concentration dependent, which limits their applicability for predicting thermodynamic properties. Nevertheless, the experimental compressibility curves can be accurately reproduced by an appropriate parametrization of the Baxter adhesive model, indicating the model provides a semiempirical equation of state for the antibody. The results provide insights into how equations of state can be improved for antibodies by accounting for their anisotropic shapes.	Aug 2017

Instruments / Technical Area

Publication Details

Published

Actions

B21-High Throughput SAXS

Coarse-grained modeling of antibodies from small-angle scattering profiles