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Coherent X-ray Diffractive Imaging of Protein Crystals

DOI: 10.1107/S0909049508029439 DOI Help
PMID: 18955763 PMID Help

Authors: S. Boutet (University of Illinois) , I. K. Robinson (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Synchrotron Radiation , VOL 15 , PAGES 576-583

State: Published (Approved)
Published: November 2008

Abstract: The technique of coherent X-ray diffraction imaging (CXDI) has recently shown great promise for the study of inorganic nanocrystals. In this work the CXDI method has been applied to the study of micrometer-size protein crystals. Finely sampled diffraction patterns of single crystals were measured and iterative phase-retrieval algorithms were used to reconstruct the two-dimensional shape of the crystal. The density maps have limited reproducibility because of radiation damage, but show clear evidence for crystal facets. Qualitative analysis of a number of single-crystal diffraction peaks indicates the presence of inward surface contraction on 2 ┬Ám size crystals. A survey of several hundred diffraction patterns yielded a number of examples with dramatic single-sided streaks, for which a plausible model is constructed.

Journal Keywords: Coherent X-Ray Diffractive Imaging; Protein Crystals; Ferritin; Phase Retrieval; Surface Contraction.

Subject Areas: Physics

Instruments: NONE-No attached Diamond beamline