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Mapping the intake of different elements in vegetal tissues by dual-energy X-ray imaging at Da Phi ne synchrotron light source

DOI: 10.1002/jemt.20538 DOI Help

Authors: L. Reale (University of L'Aquila) , J. Kaiser (Brno University of Technology) , A. Reale (University of L'Aquila) , A. Lai (ENEA) , F. Flora (ENEA) , A. Balerna (LNF-Laboratorio Dafne-luce) , M. Fanelli (Università dell'Aquila) , F. Ruggikri (Università dell'Aquila) , A. Faenov (Japan Atomic Energy Agency (JAEA)) , G. Cinque (LNF-Laboratorio Dafne-luce) , T. Pikuz (Japan Atomic Energy Agency (JAEA)) , A. Tucci (University of L'Aquila) , A. Poma (University of L'Aquila) , P. Zuppela (University of L'Aquila) , M. Liska (Brno University of Technology) , R. Malina (Brno University of Technology)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Microscopy Research And Techniques , VOL 71 (3) , PAGES 179-185

State: Published (Approved)
Published: November 2007

Abstract: This article reports on the first utilization of the soft X-ray beamline at the Da?ne synchrotron light source for mapping the intake of different elements in plant tissues. As a test, the method of dual-energy X-ray microradiography was applied to the investigation of the natural sulfur content in dried leaf and root samples. Our ultimate goal was to monitor the pollutant lead and its intake, which was added in controlled doses to the hydroponic medium of laboratory-controlled samples of vegetal species. The results obtained by the nondestructive X-ray radiographic analysis are compared to the values of concentrations determined by a standard chemical analysis utilizing atomic absorption spectroscopy. From this comparison the validity of the X-ray detection of heavy metals in biological samples has been confirmed. The superposition of the dual energy results on the simple planar radiography shows the representation of the pollutant intake directly on the sample structures. It should be pointed out that this method, developed here for plant root and leaves could be applied to any biological sample of interest, but the preparation and observation conditions necessitate different strategies according to the type of sample under analysis.

Journal Keywords: Dual Energy X-Ray Analysis; Intake; Biodisposable; Sulphur; Lead

Subject Areas: Biology and Bio-materials

Instruments: NONE-No attached Diamond beamline

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