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Osmotic Pressure and Aggregate Shape in BSA/Poly(Ethylene Glycol)-Lipid/Dextran Solutions

DOI: 10.1016/j.bpc.2008.01.004 DOI Help

Authors: V. Castelletto (University of Reading) , L. A. Clifton (University of Reading) , R. J. Green (University of Reading) , I. W. Ian Hamley (University of Reading)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Biophysical Chemistry , VOL 134 , PAGES 34-38

State: Published (Approved)
Published: January 2008

Abstract: In this work we study the colloidal osmotic pressure (COP) and aggregate shape in phosphate saline buffer solutions (pH 7.4) containing bovine serum albumin (BSA), poly(ethylene glycol) lipid (PEG2000-PE) and Dextran (Dx). Dx was added to the BSA/PEG2000-PE system in order to increase the COP of the solution to levels comparable to the COP of healthy adults, with the aim of using the solution as a blood COP regulator. Dynamic light scattering and small angle X-ray scattering results shown the formation of BSA/PEG2000-PE/Dx aggregates in the solution. Osmometry results shown that the addition of Dx to the BSA/PEG2000-PE system could successfully increase the COP, through the formation of BSA/PEG2000-PE/Dx aggregates. The BSA/PEG2000-PE/Dx solutions attained COP = 15 mm Hg, representing 60% of COP measured for healthy adults.

Journal Keywords: Bitter Taste Receptor; Pre-Crystallization Screening; Saccharomyces Cerevisiae; G Protein-Coupled Receptor; Fluorescence Size-Exclusion Chromatography; Monodispersity

Subject Areas: Chemistry, Materials

Instruments: NONE-No attached Diamond beamline

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