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Anisotropic circular dichroism of light-harvesting complex II in oriented lipid bilayers: Theory meets experiment
Authors:
Parveen
Akhtar
(Hungarian Academy of Sciences; ELI-HU Nonprofit Kft.)
,
Dominik
Lindorfer
(Johannes Kepler University Linz)
,
Monika
Lingvay
(Hungarian Academy of Sciences)
,
Krzysztof
Pawlak
(Hungarian Academy of Sciences)
,
Otto
Zsiros
(Hungarian Academy of Sciences)
,
Giuliano
Siligardi
(Diamond Light Source)
,
Tamas
Javorfi
(Diamond Light Source)
,
Márta
Dorogi
(Hungarian Academy of Sciences)
,
Bettina
Ughy
(Hungarian Academy of Sciences)
,
Gyozo
Garab
(Hungarian Academy of Sciences; University of Ostrava)
,
Thomas
Renger
(Johannes Kepler University Linz)
,
Petar H.
Lambrev
(Hungarian Academy of Sciences)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
The Journal Of Physical Chemistry B
State:
Published (Approved)
Published:
January 2019
Diamond Proposal Number(s):
15094
,
16232
,
17698
,
19120
,
20644
Abstract: Anisotropic circular dichroism (ACD) spectroscopy of macroscopically aligned molecules reveals additional information about their excited states that is lost in the CD of randomly-oriented solutions. ACD spectra of light-harvesting complex II (LHCII)—the main peripheral antenna of photosystem II in plants—in oriented lipid bilayers, were recorded from the far-UV to the visible wavelength region. The ACD spectra show a drastically enhanced magnitude and level of detail compared to the isotropic CD spectra, resolving a greater number of bands and weak optical transitions. Exciton calculations show that the spectral features in the chlorophyll Qy region are well reproduced by an existing Hamiltonian for LHCII, providing further evidence for the identity of energy sinks at chlorophylls a603 and a610 in the stromal layer and chlorophylls a604 and a613 in the luminal layer. We propose ACD spectroscopy to be a valuable tool linking the three-dimensional structure and the photophysical properties of pigment–protein complexes.
Journal Keywords: photosynthetic complexes; model membranes; optical spectroscopy; excitonic CD; exciton theory; oriented CD
Diamond Keywords: Photosynthesis
Subject Areas:
Chemistry
Instruments:
B23-Circular Dichroism
Added On:
10/01/2019 11:16
Discipline Tags:
Physical Chemistry
Chemistry
Technical Tags:
Spectroscopy
Circular Dichroism (CD)