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The cell cycle regulator GpsB functions as cytosolic adaptor for multiple cell wall enzymes

DOI: 10.1038/s41467-018-08056-2 DOI Help

Authors: Robert M. Cleverley (University of Newcastle) , Zoe J. Rutter (University of Newcastle) , Jeanine Rismondo (Robert Koch Institute) , Federico Corona (Università di Cagliari) , Ho-ching Tiffany Tsui (Indiana University Bloomington) , Fuad A. Alatawi (University of Newcastle) , Richard A. Daniel (University of Newcastle) , Sven Halbedel (Robert Koch Institute) , Orietta Massidda (Università di Cagliari) , Malcolm E. Winkler (University Bloomington) , Richard J. Lewis (University of Newcastle)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Communications , VOL 10

State: Published (Approved)
Published: January 2019
Diamond Proposal Number(s): 9948 , 13587

Open Access Open Access

Abstract: Bacterial growth and cell division requires precise spatiotemporal regulation of the synthesis and remodelling of the peptidoglycan layer that surrounds the cytoplasmic membrane. GpsB is a cytosolic protein that affects cell wall synthesis by binding cytoplasmic mini-domains of peptidoglycan synthases to ensure their correct subcellular localisation. Here, we describe critical structural features for the interaction of GpsB with peptidoglycan synthases from three bacterial species (Bacillus subtilis, Listeria monocytogenes and Streptococcus pneumoniae) and suggest their importance for cell wall growth and viability in L. monocytogenes and S. pneumoniae. We use these structural motifs to identify novel partners of GpsB in B. subtilis and extend the members of the GpsB interactome in all three bacterial species. Our results support that GpsB functions as an adaptor protein that mediates the interaction between membrane proteins, scaffolding proteins, signalling proteins and enzymes to generate larger protein complexes at specific sites in a bacterial cell cycle-dependent manner.

Journal Keywords: Bacterial structural biology; Cellular microbiology; X-ray crystallography

Subject Areas: Biology and Bio-materials

Instruments: I03-Macromolecular Crystallography , I04-Macromolecular Crystallography , I24-Microfocus Macromolecular Crystallography