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Design, 3D printing and validation of a novel low-cost high-capacity sitting-drop bridge for protein crystallization
DOI:
10.1107/S1600576718017545
Authors:
Sandeep K.
Talapatra
(University College London)
,
Matthew R.
Penny
(University College London)
,
Stephen T.
Hilton
(University College London)
,
Frank
Kozielski
(University College London)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Journal Of Applied Crystallography
, VOL 52
, PAGES 171 - 174
State:
Published (Approved)
Published:
February 2019
Diamond Proposal Number(s):
17201
Abstract: Sitting-drop protein crystallization is not used as commonly as the hanging-drop method for crystal optimization owing to the limitations of commercially available sitting-drop bridges, particularly when they are used in conjunction with 24-well crystallization plates. The commercially available sitting-drop bridge, containing space for only a single drop, restricts their wider use. Proteins that preferentially crystallize under sitting-drop conditions therefore require more work, time and resources for their optimization. In response to these limitations, and using 3D printing, a new sitting-drop bridge has been designed and developed, where five crystallization drops can be placed simultaneously in each well of a 24-well crystallization plate. This significantly simplifies the process and increases the potential of sitting drops in crystal optimization, reducing costs and hence overcoming the limitations of current approaches.
Journal Keywords: protein crystallization; sitting-drop crystallization; hanging-drop crystallization; vapour-diffusion crystallization; microbridges; 3D printing
Subject Areas:
Technique Development,
Biology and Bio-materials
Instruments:
I24-Microfocus Macromolecular Crystallography