Article Metrics


Online attention

Identification and characterization of cytochrome P450 1232A24 and 1232F1 from Arthrobacter sp. and their role in the metabolic pathway of papaverine

DOI: 10.1093/jb/mvz010 DOI Help

Authors: Jan M. Klenk (University of Stuttgart) , Max-philipp Fischer (University of Stuttgart) , Paulina Dubiel (University of York) , Mahima Sharma (University of York) , Benjamin Rowlinson (University of York) , Gideon Grogan (University of York) , Bernhard Hauer (University of Stuttgart)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: The Journal Of Biochemistry

State: Published (Approved)
Published: February 2019
Diamond Proposal Number(s): 9948

Abstract: Cytochrome P450 monooxygenases (P450s) play crucial roles in the cell metabolism and provide an unsurpassed diversity of catalyzed reactions. Here, we report the identification and biochemical characterization of two P450s from Arthrobacter sp., a gram-positive organism known to degrade the opium alkaloid papaverine. Combining phylogenetic and genomic analysis suggested physiological roles for P450s in metabolism, and revealed potential gene clusters with redox partners facilitating the reconstitution of the P450 activities in vitro. CYP1232F1 catalyzes the para demethylation of 3,4-dimethoxyphenylacetic acid to homovanillic acid while CYP1232A24 continues demethylation to 3,4-dihydroxyphenylacetic acid. Interestingly, the latter enzyme is also able to perform both demethylation steps with preference for the meta position. The crystal structure of CYP1232A24, which shares only 29% identity to previous published structures of P450s helped to rationalize the preferred demethylation specificity for the meta position and also the broader substrate specificity profile. In addition to the detailed characterization of the two P450s using their physiological redox partners, we report the construction of a highly-active whole-cell E. coli biocatalyst expressing CYP1232A24, which formed up to 1.77 g l−1 3,4-dihydroxyphenylacetic acid. Our results revealed the P450s’ role in the metabolic pathway of papaverine enabling further investigation and application of these biocatalysts.

Journal Keywords: Arthrobacter sp; biochemical characterization; crystal structure; cytochrome P450; metabolism

Subject Areas: Biology and Bio-materials, Chemistry

Instruments: I04-1-Macromolecular Crystallography (fixed wavelength)

Discipline Tags:

Technical Tags: