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Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer’s and Pick’s diseases

DOI: 10.7554/eLife.43584 DOI Help

Authors: Wenjuan Zhang (MRC Laboratory of Molecular Biology) , Benjamin Falcon (MRC Laboratory of Molecular Biology) , Alexey G. Murzin (MRC Laboratory of Molecular Biology) , Juan Fan (MRC Laboratory of Molecular Biology) , R. Anthony Crowther (MRC Laboratory of Molecular Biology) , Michel Goedert (MRC Laboratory of Molecular Biology) , Sjors H. W. Scheres (MRC Laboratory of Molecular Biology)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Elife , VOL 8

State: Published (Approved)
Published: February 2019
Diamond Proposal Number(s): 17434

Open Access Open Access

Abstract: Assembly of microtubule-associated protein tau into filamentous inclusions underlies a range of neurodegenerative diseases. Tau filaments adopt different conformations in Alzheimer’s and Pick’s diseases. Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin. 2N4R tau assembles into multiple types of filaments, and the structures of three types reveal similar ‘kinked hairpin’ folds, in which the second and third repeats pack against each other. 2N3R tau filaments are structurally homogeneous, and adopt a dimeric core, where the third repeats of two tau molecules pack in a parallel manner. The heparin-induced tau filaments differ from those of Alzheimer’s or Pick’s disease, which have larger cores with different repeat compositions. Our results illustrate the structural versatility of amyloid filaments, and raise questions about the relevance of in vitro assembly.

Subject Areas: Biology and Bio-materials

Diamond Offline Facilities: Electron Bio-Imaging Centre (eBIC)
Instruments: Krios I-Titan Krios I at Diamond

Documents:
elife-43584-v2 (1).pdf