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MM-131, a bispecific anti-Met/EpCAM mAb, inhibits HGF-dependent and HGF-independent Met signaling through concurrent binding to EpCAM

DOI: 10.1073/pnas.1819085116 DOI Help

Authors: Jessica B. Casaletto (Merrimack Pharmaceuticals, Inc) , Melissa L. Geddie (Merrimack Pharmaceuticals, Inc) , Adnan O. Abu-Yousif (Merrimack Pharmaceuticals, Inc) , Kristina Masson (Merrimack Pharmaceuticals, Inc) , Aaron Fulgham (Merrimack Pharmaceuticals, Inc) , Antoine Boudot (Merrimack Pharmaceuticals, Inc) , Tim Maiwald (Merrimack Pharmaceuticals, Inc) , Jeffrey D. Kearns (Merrimack Pharmaceuticals, Inc) , Neeraj Kohli (Merrimack Pharmaceuticals, Inc) , Stephen Su (Merrimack Pharmaceuticals, Inc) , Maja Razlog (Merrimack Pharmaceuticals, Inc) , Andreas Raue (Merrimack Pharmaceuticals, Inc) , Ashish Kalra (Merrimack Pharmaceuticals, Inc) , Maria HÃ¥kansson (SARomics Biostructures AB) , Derek T. Logan (SARomics Biostructures AB) , Martin Welin (SARomics Biostructures AB) , Shrikanta Chattopadhyay (Merrimack Pharmaceuticals, Inc) , Brian D. Harms (Merrimack Pharmaceuticals, Inc) , Ulrik B. Nielsen (Merrimack Pharmaceuticals, Inc) , Birgit Schoeberl (Merrimack Pharmaceuticals, Inc) , Alexey A. Lugovskoy (Merrimack Pharmaceuticals, Inc) , Gavin Macbeath (Merrimack Pharmaceuticals, Inc)
Co-authored by industrial partner: Yes

Type: Journal Paper
Journal: Proceedings Of The National Academy Of Sciences , VOL 10

State: Published (Approved)
Published: March 2019
Diamond Proposal Number(s): 12427

Abstract: Activation of the Met receptor tyrosine kinase, either by its ligand, hepatocyte growth factor (HGF), or via ligand-independent mechanisms, such as MET amplification or receptor overexpression, has been implicated in driving tumor proliferation, metastasis, and resistance to therapy. Clinical development of Met-targeted antibodies has been challenging, however, as bivalent antibodies exhibit agonistic properties, whereas monovalent antibodies lack potency and the capacity to down-regulate Met. Through computational modeling, we found that the potency of a monovalent antibody targeting Met could be dramatically improved by introducing a second binding site that recognizes an unrelated, highly expressed antigen on the tumor cell surface. Guided by this prediction, we engineered MM-131, a bispecific antibody that is monovalent for both Met and epithelial cell adhesion molecule (EpCAM). MM-131 is a purely antagonistic antibody that blocks ligand-dependent and ligand-independent Met signaling by inhibiting HGF binding to Met and inducing receptor down-regulation. Together, these mechanisms lead to inhibition of proliferation in Met-driven cancer cells, inhibition of HGF-mediated cancer cell migration, and inhibition of tumor growth in HGF-dependent and -independent mouse xenograft models. Consistent with its design, MM-131 is more potent in EpCAM-high cells than in EpCAM-low cells, and its potency decreases when EpCAM levels are reduced by RNAi. Evaluation of Met, EpCAM, and HGF levels in human tumor samples reveals that EpCAM is expressed at high levels in a wide range of Met-positive tumor types, suggesting a broad opportunity for clinical development of MM-131.

Journal Keywords: bispecific antibody; Met; HGF; EpCAM; cancer

Diamond Keywords: Immunotherapy

Subject Areas: Biology and Bio-materials, Medicine

Instruments: I02-Macromolecular Crystallography

Added On: 09/04/2019 12:11

Discipline Tags:

Non-Communicable Diseases Health & Wellbeing Cancer Structural biology Drug Discovery Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)