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Accessibility to peptidoglycan is important for the recognition of gram-positive bacteria in Drosophila

DOI: 10.1016/j.celrep.2019.04.103 DOI Help

Authors: Filipa Vaz (University of Oxford; Universidade Nova de Lisboa) , Ilias Kounatidis (University of Oxford; Diamond Light Source) , Gonçalo Covas (Universidade Nova de Lisboa) , Richard M. Parton (University of Oxford) , Maria Harkiolaki (Diamond Light Source) , Ilan Davis (University of Oxford) , Sergio Raposo Filipe (Universidade Nova de Lisboa) , Petros Ligoxygakis (University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Cell Reports , VOL 27 , PAGES 2480 - 2492.e6

State: Published (Approved)
Published: May 2019

Open Access Open Access

Abstract: In Drosophila, it is thought that peptidoglycan recognition proteins (PGRPs) SA and LC structurally discriminate between bacterial peptidoglycans with lysine (Lys) or diaminopimelic (DAP) acid, respectively, thus inducing differential antimicrobial transcription response. Here, we find that accessibility to PG at the cell wall plays a central role in immunity to infection. When wall teichoic acids (WTAs) are genetically removed from S. aureus (Lys type) and Bacillus subtilis (DAP type), thus increasing accessibility, the binding of both PGRPs to either bacterium is increased. PGRP-SA and -LC double mutant flies are more susceptible to infection with both WTA-less bacteria. In addition, WTA-less bacteria grow better in PGRP-SA/-LC double mutant flies. Finally, infection with WTA-less bacteria abolishes any differential activation of downstream antimicrobial transcription. Our results indicate that accessibility to cell wall PG is a major factor in PGRP-mediated immunity and may be the cause for discrimination between classes of pathogens.

Journal Keywords: Drosophila; PGRPs; innate immunity; S. aureus; B. subtilis; TagO; peptidoglycan

Subject Areas: Biology and Bio-materials


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