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Direct measurement of X-ray-induced heating of microcrystals

DOI: 10.1107/S1600577519003849 DOI Help

Authors: Anna Warren (Diamond Light Source) , Danny Axford (Diamond Light Source) , Robin L. Owen (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Synchrotron Radiation , VOL 26

State: Published (Approved)
Published: July 2019

Open Access Open Access

Abstract: Temperature control is a key aspect of macromolecular crystallography, with the technique of cryocooling routinely being used to mitigate X-ray-induced damage. Beam-induced heating could cause the temperature of crystals to rise above the glass transition temperature, greatly increasing the rate of damage. X-ray-induced heating of ruby crystals of 20–40 µm in size has been quantified non-invasively by monitoring the emission wavelengths of X-ray-induced fluorescence during exposure to the X-ray beam. For the beam sizes and dose rates typically used in macromolecular crystallography, the temperature rises are of the order of 20 K. The temperature changes observed are compared with models in the literature and can be used as a validation tool for future models.

Journal Keywords: X-ray-induced beam heating; fluorescence; cryocooling

Subject Areas: Technique Development


Instruments: I24-Microfocus Macromolecular Crystallography

Documents:
gm5057.pdf