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Selection of biophysical methods for characterisation of membrane proteins

DOI: 10.3390/ijms20102605 DOI Help

Authors: Tristan O. C. Kwan (National Physical Laboratory; Research Complex at Harwell) , Rosana Reis (National Physical Laboratory; Research Complex at Harwell) , Giuliano Siligardi (Diamond Light Source) , Rohanah Hussain (Diamond Light Source) , Harish Cheruvara (Research Complex at Harwell; Diamond Light Source) , Isabel Moraes (National Physical Laboratory; Research Complex at Harwell)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: International Journal Of Molecular Sciences , VOL 20

State: Published (Approved)
Published: May 2019
Diamond Proposal Number(s): 12182

Open Access Open Access

Abstract: Over the years, there have been many developments and advances in the field of integral membrane protein research. As important pharmaceutical targets, it is paramount to understand the mechanisms of action that govern their structure–function relationships. However, the study of integral membrane proteins is still incredibly challenging, mostly due to their low expression and instability once extracted from the native biological membrane. Nevertheless, milligrams of pure, stable, and functional protein are always required for biochemical and structural studies. Many modern biophysical tools are available today that provide critical information regarding to the characterisation and behaviour of integral membrane proteins in solution. These biophysical approaches play an important role in both basic research and in early-stage drug discovery processes. In this review, it is not our objective to present a comprehensive list of all existing biophysical methods, but a selection of the most useful and easily applied to basic integral membrane protein research.

Journal Keywords: membrane proteins; biophysics; dynamic light scattering; circular dichroism; SEC-MALS; MIR; LCP-FRAP

Subject Areas: Biology and Bio-materials, Medicine

Diamond Offline Facilities: Membrane Protein Laboratory (MPL)
Instruments: B23-Circular Dichroism

ijms-20-02605 (1).pdf