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Importance of potassium ions for ribosome structure and function revealed by long-wavelength X-ray diffraction

DOI: 10.1038/s41467-019-10409-4 DOI Help

Authors: Alexey Rozov (Institut de Génétique et de Biologie Moléculaire et Cellulaire; Centre National de la Recherche Scientifique; Institut National de la Santé et de la Recherche Médicale; Université de Strasbourg; RiboStruct) , Iskander Khusainov (Institut de Génétique et de Biologie Moléculaire et Cellulaire; Centre National de la Recherche Scientifique; Institut National de la Santé et de la Recherche Médicale; Université de Strasbourg; Kazan Federal University) , Kamel El Omari (Diamond Light Source; Research Complex at Harwell) , Ramona Duman (Diamond Light Source; Research Complex at Harwell) , Vitaliy Mykhaylyk (Diamond Light Source) , Marat Yusupov (Institut de Génétique et de Biologie Moléculaire et Cellulaire; Centre National de la Recherche Scientifique; Institut National de la Santé et de la Recherche Médicale; Université de Strasbourg; Kazan Federal University) , Eric Westhof (Université de Strasbourg, CNRS) , Armin Wagner (Diamond Light Source) , Gulnara Yusupova (Institut de Génétique et de Biologie Moléculaire et Cellulaire; Centre National de la Recherche Scientifique; Institut National de la Santé et de la Recherche Médicale; Université de Strasbourg)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Communications

State: Published (Approved)
Published: June 2019

Open Access Open Access

Abstract: The ribosome, the largest RNA-containing macromolecular machinery in cells, requires metal ions not only to maintain its three-dimensional fold but also to perform protein synthesis. Despite the vast biochemical data regarding the importance of metal ions for efficient protein synthesis and the increasing number of ribosome structures solved by X-ray crystallography or cryo-electron microscopy, the assignment of metal ions within the ribosome remains elusive due to methodological limitations. Here we present extensive experimental data on the potassium composition and environment in two structures of functional ribosome complexes obtained by measurement of the potassium anomalous signal at the K-edge, derived from long-wavelength X-ray diffraction data. We elucidate the role of potassium ions in protein synthesis at the three-dimensional level, most notably, in the environment of the ribosome functional decoding and peptidyl transferase centers. Our data expand the fundamental knowledge of the mechanism of ribosome function and structural integrity.

Journal Keywords: Potassium; Ribosome; X-ray crystallography

Subject Areas: Biology and Bio-materials, Chemistry


Instruments: I23-Long wavelength MX

Documents:
s41467-019-10409-4.pdf