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A ubiquitin-binding domain that binds a structural fold distinct from that of ubiquitin

DOI: 10.1016/j.str.2019.05.003 DOI Help

Authors: Michael Lim (The Francis Crick Institute (LIF)) , Joseph A. Newman (Structural Genomics Consortium and Target Discovery Institute, University of Oxford) , Hannah L. Williams (The Francis Crick Institute) , Laura Masino (The Francis Crick Institute) , Hazel Aitkenhead (Structural Genomics Consortium and Target Discovery Institute, University of Oxford) , Angeline E. Gravard (Structural Genomics Consortium and Target Discovery Institute, University of Oxford) , Opher Gileadi (Structural Genomics Consortium and Target Discovery Institute, University of Oxford) , Jesper Q. Svejstrup (The Francis Crick Institute)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Structure

State: Published (Approved)
Published: June 2019
Diamond Proposal Number(s): 19301 , 15433

Abstract: Ubiquitylation, the posttranslational linkage of ubiquitin moieties to lysines in target proteins, helps regulate a myriad of biological processes. Ubiquitin, and sometimes ubiquitin-homology domains, are recognized by ubiquitin-binding domains, including CUE domains. CUE domains are thus generally thought to function by mediating interactions with ubiquitylated proteins. The chromatin remodeler, SMARCAD1, interacts with KAP1, a transcriptional corepressor. The SMARCAD1-KAP1 interaction is direct and involves the first SMARCAD1 CUE domain (CUE1) and the RBCC domain of KAP1. Here, we present a structural model of the KAP1 RBCC-SMARCAD1 CUE1 complex based on X-ray crystallography. Remarkably, CUE1, a canonical CUE domain, recognizes a cluster of exposed hydrophobic and surrounding charged/amphipathic residues on KAP1, which are presented in the context of a coiled-coil domain, not in a structure resembling ubiquitin. Together, these data suggest that CUE domains may have a wider function than simply recognizing ubiquitin and the ubiquitin-fold.

Journal Keywords: ubiquitin; ubiquitin-binding domain; CUE domain; UBA domain; KAP1; TRIM28; TIF1β; SMARCAD1

Subject Areas: Biology and Bio-materials


Instruments: I03-Macromolecular Crystallography , I04-1-Macromolecular Crystallography (fixed wavelength)