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Designer artificial membrane binding proteins to direct stem cells to the myocardium

DOI: 10.1039/C9SC02650A DOI Help

Authors: Wenjin Xiao (University of Bristol) , Thomas I. P. Green (University of Bristol) , Xiaowen Liang (The University of Queensland) , Rosalia Cuahtecontzi Delint (University of Bristol) , Guillaume Perry (Sorbonne Université) , Michael S. Roberts (he University of Queensland; University of South Australia,) , Kristian Le Vay (University of Bristol) , Catherine R. Back (University of Bristol) , Raimomdo Ascione (University of Bristol) , Haolu Wang (The University of Queensland) , Paul R. Race (University of Bristol) , Adam W. Perriman (University of Bristol)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Chemical Science , VOL 20

State: Published (Approved)
Published: July 2019

Open Access Open Access

Abstract: We present a new cell membrane modification methodology where the inherent heart tissue homing properties of the infectious bacteria Streptococcus gordonii are transferred to human stem cells. This is achieved via the rational design of a chimeric protein–polymer surfactant cell membrane binding construct, comprising the cardiac fibronectin (Fn) binding domain of the bacterial adhesin protein CshA fused to a supercharged protein. Significantly, the protein–polymer surfactant hybrid spontaneously inserts into the plasma membrane of stem cells without cytotoxicity, instilling the cells with a high affinity for immobilized fibronectin. Moreover, we show that this cell membrane reengineering approach significantly improves retention and homing of stem cells delivered either intracardially or intravenously to the myocardium in a mouse model.

Subject Areas: Biology and Bio-materials

Instruments: B21-High Throughput SAXS , B23-Circular Dichroism


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