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Plant-made nervous necrosis virus-like particles protect fish against disease
Authors:
Johanna
Marsian
(John Innes Centre)
,
Daniel L.
Hurdiss
(University of Leeds)
,
Neil A.
Ranson
(University of Leeds)
,
Anneli
Ritala
(VTT Technical Research Centre of Finland)
,
Richard
Paley
(Centre for Environment Fisheries and Aquaculture Science (Cefas))
,
Irene
Cano
(Centre for Environment Fisheries and Aquaculture Science (Cefas))
,
George P.
Lomonossoff
(John Innes Centre)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Frontiers In Plant Science
, VOL 10
State:
Published (Approved)
Published:
July 2019
Diamond Proposal Number(s):
13452

Abstract: Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.
Journal Keywords: Atlantic cod nervous necrosis virus; virus-like particles; transient expression; sea bass; protective immunity
Diamond Keywords: Viral Nervous Necrosis; Viruses
Subject Areas:
Biology and Bio-materials
Diamond Offline Facilities:
Electron Bio-Imaging Centre (eBIC)
Instruments:
Krios I-Titan Krios I at Diamond
Added On:
30/07/2019 14:55
Documents:
fpls1000880.pdf
Discipline Tags:
Plant science
Pathogens
Structural biology
Drug Discovery
Life Sciences & Biotech
Veterinary Medicine
Technical Tags:
Microscopy
Electron Microscopy (EM)
Cryo Electron Microscopy (Cryo EM)