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Plant-made nervous necrosis virus-like particles protect fish against disease

DOI: 10.3389/fpls.2019.00880 DOI Help

Authors: Johanna Marsian (John Innes Centre) , Daniel L. Hurdiss (University of Leeds) , Neil A. Ranson (University of Leeds) , Anneli Ritala (VTT Technical Research Centre of Finland) , Richard Paley (Centre for Environment Fisheries and Aquaculture Science (Cefas)) , Irene Cano (Centre for Environment Fisheries and Aquaculture Science (Cefas)) , George P. Lomonossoff (John Innes Centre)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Frontiers In Plant Science , VOL 10

State: Published (Approved)
Published: July 2019
Diamond Proposal Number(s): 13452

Open Access Open Access

Abstract: Virus-like particles (VLPs) of the fish virus, Atlantic Cod Nervous necrosis virus (ACNNV), were successfully produced by transient expression of the coat protein in Nicotiana benthamiana plants. VLPs could also be produced in transgenic tobacco BY-2 cells. The protein extracted from plants self-assembled into T = 3 particles, that appeared to be morphologically similar to previously analyzed NNV VLPs when analyzed by high resolution cryo-electron microscopy. Administration of the plant-produced VLPs to sea bass (Dicentrarchus labrax) showed that they could protect the fish against subsequent virus challenge, indicating that plant-produced vaccines may have a substantial future role in aquaculture.

Journal Keywords: Atlantic cod nervous necrosis virus; virus-like particles; transient expression; sea bass; protective immunity

Diamond Keywords: Viral Nervous Necrosis; Viruses

Subject Areas: Biology and Bio-materials

Diamond Offline Facilities: Electron Bio-Imaging Centre (eBIC)
Instruments: Krios I-Titan Krios I at Diamond

Added On: 30/07/2019 14:55


Discipline Tags:

Plant science Pathogens Structural biology Drug Discovery Life Sciences & Biotech Veterinary Medicine

Technical Tags:

Microscopy Electron Microscopy (EM) Cryo Electron Microscopy (Cryo EM)