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Nuclear uptake of gold nanoparticles deduced using dual‐angle X‐ray fluorescence mapping

DOI: 10.1002/ppsc.201900140 DOI Help

Authors: Aaron Mcculloch (Queen's University of Belfast) , Lindsey Bennie (Queen's University of Belfast) , Jonathan A. Coulter (Queen's University of Belfast) , Helen O. Mccarthy (Queen's University Belfast) , Brendan Dromey (Queen's University Belfast) , David R. Grimes (Queen's University Belfast) , Paul Quinn (Diamond Light Source) , Balder Villagomez-Bernabe (Queen's University of Belfast) , Frederick Currell (The University of Manchester)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Particle & Particle Systems Characterization , VOL 6

State: Published (Approved)
Published: July 2019
Diamond Proposal Number(s): 16931 , 17932

Open Access Open Access

Abstract: Studies into the cell nucleus' incorporation of gold nanoparticles (AuNPs) are often limited by ambiguities arising from conventional imaging techniques. Indeed, it is suggested that to date there is no unambiguous imaging evidence for such uptake in whole cells, particularly at the single nanoparticle level. This shortcoming in understanding exists despite the nucleus being the most important subcellular compartment in eukaryotes and gold being the most commonly used metal nanoparticle in medical applications. Here, dual‐angle X‐ray flouresence is used to show individually resolved nanoparticles within the cell nucleus, finding them to be well separated and 79% of the intranuclear population to be monodispersed. These findings have important implications for nanomedicine, illustrated here through a specific exemplar of the predicted enhancement of radiation effects arising from the observed AuNPs, finding intranuclear dose enhancements spanning nearly five orders of magnitude.

Journal Keywords: gold nanoparticles; nanomedicine; nuclear uptake; X‐ray fluorescence; X‐ray microscopy

Subject Areas: Physics, Materials, Medicine

Instruments: I14-Hard X-ray Nanoprobe


Discipline Tags:

Drug Delivery Physics Health & Wellbeing Material Sciences Nanoscience/Nanotechnology Biophysics Life Sciences & Biotech

Technical Tags:

Imaging X-ray Fluorescence (XRF)