Reducing sample consumption for serial crystallography using acoustic drop ejection

DOI: 10.1107/S1600577519009329

Authors: Bradley Davy (Diamond Light Source) , Danny Axford (Diamond Light Source) , John H. Beale (Diamond Light Source) , Agata Butryn (Diamond Light Source) , Peter Docker (Diamond Light Source) , Ali Ebrahim (University of Essex; Diamond Light Source) , Gabriel Leen (PolyPico Technologies Ltd; University of Limerick) , Allen M. Orville (Diamond Light Source; Research Complex at Harwell) , Robin L. Owen (Diamond Light Source) , Pierre Aller (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Synchrotron Radiation , VOL 26

State: Published (Approved)
Published: September 2019
Diamond Proposal Number(s): 14493 , 19458

Abstract: Efficient sample delivery is an essential aspect of serial crystallography at both synchrotrons and X-ray free-electron lasers. Rastering fixed target chips through the X-ray beam is an efficient method for serial delivery from the perspectives of both sample consumption and beam time usage. Here, an approach for loading fixed targets using acoustic drop ejection is presented that does not compromise crystal quality, can reduce sample consumption by more than an order of magnitude and allows serial diffraction to be collected from a larger proportion of the crystals in the slurry.

Journal Keywords: serial crystallography; sample delivery; fixed targets; acoustic dispensing

Subject Areas: Technique Development


Instruments: I24-Microfocus Macromolecular Crystallography

Documents:
gb5089.pdf