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Reducing sample consumption for serial crystallography using acoustic drop ejection
DOI:
10.1107/S1600577519009329
Authors:
Bradley
Davy
(Diamond Light Source)
,
Danny
Axford
(Diamond Light Source)
,
John H.
Beale
(Diamond Light Source)
,
Agata
Butryn
(Diamond Light Source)
,
Peter
Docker
(Diamond Light Source)
,
Ali
Ebrahim
(University of Essex; Diamond Light Source)
,
Gabriel
Leen
(PolyPico Technologies Ltd; University of Limerick)
,
Allen M.
Orville
(Diamond Light Source; Research Complex at Harwell)
,
Robin L.
Owen
(Diamond Light Source)
,
Pierre
Aller
(Diamond Light Source)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Journal Of Synchrotron Radiation
, VOL 26
State:
Published (Approved)
Published:
September 2019
Diamond Proposal Number(s):
14493
,
19458

Abstract: Efficient sample delivery is an essential aspect of serial crystallography at both synchrotrons and X-ray free-electron lasers. Rastering fixed target chips through the X-ray beam is an efficient method for serial delivery from the perspectives of both sample consumption and beam time usage. Here, an approach for loading fixed targets using acoustic drop ejection is presented that does not compromise crystal quality, can reduce sample consumption by more than an order of magnitude and allows serial diffraction to be collected from a larger proportion of the crystals in the slurry.
Journal Keywords: serial crystallography; sample delivery; fixed targets; acoustic dispensing
Subject Areas:
Technique Development
Instruments:
I24-Microfocus Macromolecular Crystallography
Added On:
20/08/2019 11:07
Documents:
gb5089.pdf
Discipline Tags:
Technique Development - Life Sciences & Biotech
Life Sciences & Biotech
Technical Tags:
Diffraction
Macromolecular Crystallography (MX)