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Non-contact universal sample presentation for room temperature macromolecular crystallography using acoustic levitation

DOI: 10.1038/s41598-019-48612-4 DOI Help

Authors: R. H. Morris (Nottingham Trent University) , E. R. Dye (Nottingham Trent University) , D. Axford (Diamond Light Source) , M. I. Newton (Nottingham Trent University) , J. H. Beale (Diamond Light Source) , P. T. Docker (Diamond Light Source)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Scientific Reports , VOL 9

State: Published (Approved)
Published: August 2019

Open Access Open Access

Abstract: Macromolecular Crystallography is a powerful and valuable technique to assess protein structures. Samples are commonly cryogenically cooled to minimise radiation damage effects from the X-ray beam, but low temperatures hinder normal protein functions and this procedure can introduce structural artefacts. Previous experiments utilising acoustic levitation for beamline science have focused on Langevin horns which deliver significant power to the confined droplet and are complex to set up accurately. In this work, the low power, portable TinyLev acoustic levitation system is used in combination with an approach to dispense and contain droplets, free of physical sample support to aid protein crystallography experiments. This method facilitates efficient X-ray data acquisition in ambient conditions compatible with dynamic studies. Levitated samples remain free of interference from fixed sample mounts, receive negligible heating, do not suffer significant evaporation and since the system occupies a small volume, can be readily installed at other light sources.

Journal Keywords: Acoustics; Characterization and analytical techniques

Subject Areas: Technique Development, Biology and Bio-materials

Instruments: I24-Microfocus Macromolecular Crystallography

Added On: 03/09/2019 08:21


Discipline Tags:

Technique Development - Life Sciences & Biotech Structural biology Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)