Article Metrics


Online attention

Quantification of the whole lymph node vasculature based on tomography of the vessel corrosion casts

DOI: 10.1038/s41598-019-49055-7 DOI Help

Authors: M. Jafarnejad (Johns Hopkins University School of Medicine) , A. Z. Ismail (Imperial College London) , D. Duarte (Imperial College London) , C. Vyas (University of Manchester) , A. Ghahramani (The Francis Crick Institute) , D. C. Zawieja (Texas A&M Health Science Center) , C. Lo Celso (Imperial College London; The Francis Crick Institute) , G. Poologasundarampillai (University of Birmingham) , J. E. Moore (Imperial College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Scientific Reports , VOL 9

State: Published (Approved)
Published: September 2019
Diamond Proposal Number(s): 13750

Open Access Open Access

Abstract: Lymph nodes (LN) are crucial for immune function, and comprise an important interface between the blood and lymphatic systems. Blood vessels (BV) in LN are highly specialized, featuring high endothelial venules across which most of the resident lymphocytes crossed. Previous measurements of overall lymph and BV flow rates demonstrated that fluid also crosses BV walls, and that this is important for immune function. However, the spatial distribution of the BV in LN has not been quantified to the degree necessary to analyse the distribution of transmural fluid movement. In this study, we seek to quantify the spatial localization of LNBV, and to predict fluid movement across BV walls. MicroCT imaging of murine popliteal LN showed that capillaries were responsible for approximately 75% of the BV wall surface area, and that this was mostly distributed around the periphery of the node. We then modelled blood flow through the BV to obtain spatially resolved hydrostatic pressures, which were then combined with Starling’s law to predict transmural flow. Much of the total 10 nL/min transmural flow (under normal conditions) was concentrated in the periphery, corresponding closely with surface area distribution. These results provide important insights into the inner workings of LN, and provide a basis for further exploration of the role of LN flow patterns in normal and pathological functions.

Journal Keywords: Immune system; Lymphatic system

Subject Areas: Biology and Bio-materials

Instruments: I13-2-Diamond Manchester Imaging

Added On: 24/09/2019 10:55


Discipline Tags:

Life Sciences & Biotech Health & Wellbeing

Technical Tags:

Imaging Tomography