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Near-atomic resolution analysis of BipD, a component of the type III secretion system of Burkholderia pseudomallei

DOI: 10.1107/S1744309110026333 DOI Help
PMID: 20823511 PMID Help

Authors: M. Pal (University College London) , Peter Erskine (Royal Free and University College Medical School) , R. S. Gill (UCL Department of Medicine) , S, P. Wood (Centre for Amyloidosis and Acute Phase Proteins, Division of Medicine, UCL Medical School) , J. B. Cooper (UCL Department of Medicine)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology And Crystallization Communications , VOL 66 (9) , PAGES 990-993

State: Published (Approved)
Published: September 2010

Abstract: Burkholderia pseudomallei, the causative agent of melioidosis, possesses a type III protein secretion apparatus that is similar to those found in Salmonella and Shigella. A major function of these secretion systems is to inject virulence-associated proteins into target cells of the host organism. The bipD gene of B. pseudomallei encodes a secreted virulence factor that is similar in sequence and is most likely to be functionally analogous to IpaD from Shigella and SipD from Salmonella. Proteins in this family are thought to act as extracellular chaperones at the tip of the secretion needle to help the hydrophobic translocator proteins enter the target cell membrane, where they form a pore and may also link the translocon pore with the secretion needle. BipD has been crystallized in a monoclinic crystal form that diffracted X-rays to 1.5 Å resolution and the structure was refined to an R factor of 16.1% and an Rfree of 19.8% at this resolution. The putative dimer interface that was observed in previous crystal structures was retained and a larger surface area was buried in the new crystal form.

Journal Keywords: Burkholderia; Crystallography; X-Ray; Membrane; Models; Molecular; Protein; Tertiary

Subject Areas: Biology and Bio-materials

Instruments: I02-Macromolecular Crystallography

Added On: 23/09/2010 13:40

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