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The Corynebacterium glutamicum aconitase repressor: scratching around for crystals

DOI: 10.1107/S1744309110029015 DOI Help
PMID: 20823530 PMID Help

Authors: Javier García-nafría (University of York) , Meike Baumgart (Institut für Biotechnologie 1, Forschungszentrum Jülich) , Michael Bott (Institut für Biotechnologie 1, Forschungszentrum Jülich) , Anthony Wilkinson (University of York) , Keith Wilson (University of York)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology And Crystallization Communications , VOL 66(9) , PAGES 1074-1077

State: Published (Approved)
Published: August 2010

Abstract: Imperfections on the surfaces of crystallization containers are known to influence crystal formation and are thought to do so by helping to overcome the nucleation barrier. The intentional creation of imperfections has been widely applied to induce crystallization of small molecules, but has not been reported for protein crystallization. Here, the crystallization and preliminary X-ray analysis of the TetR-type aconitase repressor are reported. This regulator was the first transcription factor to be identified in the regulation of the tricarboxylic acid cycle in Corynebacterium glutamicum, an organism that is of special industrial interest and is an emerging model organism for Corynebacterineae. Successful crystallization involved introducing manual scratches on the surface of standard commercial plates, which led to a substantial improvement in crystal nucleation and quality.

Subject Areas: Biology and Bio-materials

Instruments: I02-Macromolecular Crystallography

Other Facilities: ESRF