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Allosteric regulation of a prokaryotic small Ras-like GTPase contributes to cell polarity oscillations in bacterial motility

DOI: 10.1371/journal.pbio.3000459 DOI Help

Authors: Jyoti Baranwal (Indian Institute of Science Education and Research) , Sébastien Lhospice (CNRS-Aix Marseille University) , Manil Kanade (Indian Institute of Science Education and Research) , Sukanya Chakraborty (Indian Institute of Science Education and Research) , Priyanka Rajendra Gade (Indian Institute of Science Education and Research) , Shrikant Harne (Indian Institute of Science Education and Research) , Julien Herrou (CNRS-Aix Marseille University) , Tâm Mignot (CNRS-Aix Marseille University) , Pananghat Gayathri (Indian Institute of Science Education and Research)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Plos Biology , VOL 17

State: Published (Approved)
Published: September 2019
Diamond Proposal Number(s): 14198 , 15369 , 16452 , 17251

Open Access Open Access

Abstract: Mutual gliding motility A (MglA), a small Ras-like GTPase; Mutual gliding motility B (MglB), its GTPase activating protein (GAP); and Required for Motility Response Regulator (RomR), a protein that contains a response regulator receiver domain, are major components of a GTPase-dependent biochemical oscillator that drives cell polarity reversals in the bacterium Myxococcus xanthus. We report the crystal structure of a complex of M. xanthus MglA and MglB, which reveals that the C-terminal helix (Ct-helix) from one protomer of the dimeric MglB binds to a pocket distal to the active site of MglA. MglB increases the GTPase activity of MglA by reorientation of key catalytic residues of MglA (a GAP function) combined with allosteric regulation of nucleotide exchange by the Ct-helix (a guanine nucleotide exchange factor [GEF] function). The dual GAP-GEF activities of MglB accelerate the rate of GTP hydrolysis over multiple enzymatic cycles. Consistent with its GAP and GEF activities, MglB interacts with MglA bound to either GTP or GDP. The regulation is essential for cell polarity, because deletion of the Ct-helix causes bipolar localization of MglA, MglB, and RomR, thereby causing reversal defects in M. xanthus. A bioinformatics analysis reveals the presence of Ct-helix in homologues of MglB in other bacterial phyla, suggestive of the prevalence of the allosteric mechanism among other prokaryotic small Ras-like GTPases.

Journal Keywords: Guanosine triphosphatase; Guanine nucleotide exchange factors; Crystal structure; Pathogen motility; Anisotropy; Allosteric regulation; Enzyme assays; Cell polarity

Subject Areas: Biology and Bio-materials

Instruments: I03-Macromolecular Crystallography , I04-Macromolecular Crystallography

Other Facilities: ESRF