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Analysis of glulisine crystallisation utilising phase diagrams and nucleants

DOI: 10.3390/cryst9090462 DOI Help

Authors: Yanmin Li (Imperial College London; Queen Mary University of London) , Lata Govada (Imperial College London) , Hodaya V. Solomon (Imperial College London) , Richard B. Gillis (University of Nottingham) , Gary G. Adams (University of Nottingham) , Naomi E. Chayen (Imperial College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Crystals , VOL 9

State: Published (Approved)
Published: September 2019
Diamond Proposal Number(s): 17221

Open Access Open Access

Abstract: Glulisine is a US Food and Drug Administration (FDA) approved insulin analogue, used for controlling hyperglycaemia in patients with diabetes mellitus (DM). It is fast acting which better approximates physiological insulin secretion, improving patient outcome. Crystallisation of Glulisine was analysed by its crystallisation phase diagram and nucleation-inducing materials. Both the hanging drop vapour diffusion and microbatch-under-oil methods were used and compared. We have shown that the same protein can have different solubility behaviours depending on the nature of the salt in the precipitating agent. In the case of Glulisine with magnesium formate, lowering the precipitant concentration drove the system further into supersaturation resulting in the formation of crystals and precipitation. This was the opposite effect to the usual scenario where raising the precipitant concentration leads to supersaturation. Glulisine with sodium potassium tartrate tetrahydrate (NaKT) followed the expected trend of forming crystals or precipitate at higher concentrations and clear drops at lower concentrations of the precipitant. The outcomes of crystallisation using the different crystallisation methods is also described. Glulisine was successfully crystallised and the crystals diffracted up to a resolution limit of 1.4 Å.

Journal Keywords: proteins; macromolecules; crystals; crystallization; nucleation; phase diagram; insulin; Glulisine

Diamond Keywords: Diabetes

Subject Areas: Biology and Bio-materials, Medicine

Instruments: I04-1-Macromolecular Crystallography (fixed wavelength)

Added On: 02/10/2019 15:22

Discipline Tags:

Life Sciences & Biotech Structural biology

Technical Tags:

Diffraction Macromolecular Crystallography (MX)