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Structure of the trypanosome transferrin receptor reveals mechanisms of ligand recognition and immune evasion

DOI: 10.1038/s41564-019-0589-0 DOI Help

Authors: Camilla E. Trevor (University of Cambridge; University of Oxford; AstraZeneca R&D) , Andrea L. Gonzalez-Munoz (AstraZeneca R&D) , Olivia J. S. Macleod (University of Cambridge) , Peter G. Woodcock (University of Oxford) , Steven Rust (AstraZeneca R&D) , Tristan J. Vaughan (AstraZeneca R&D) , Elspeth F. Garman (University of Oxford) , Ralph Minter (AstraZeneca R&D) , Mark Carrington (University of Cambridge) , Matthew K. Higgins (University of Oxford)
Co-authored by industrial partner: Yes

Type: Journal Paper
Journal: Nature Microbiology , VOL 1

State: Published (Approved)
Published: October 2019
Diamond Proposal Number(s): 12346

Abstract: To maintain prolonged infection of mammals, African trypanosomes have evolved remarkable surface coats and a system of antigenic variation. Within these coats are receptors for macromolecular nutrients such as transferrin. These must be accessible to their ligands but must not confer susceptibility to immunoglobulin-mediated attack. Trypanosomes have a wide host range and their receptors must also bind ligands from diverse species. To understand how these requirements are achieved, in the context of transferrin uptake, we determined the structure of a Trypanosoma brucei transferrin receptor in complex with human transferrin, showing how this heterodimeric receptor presents a large asymmetric ligand-binding platform. The trypanosome genome contains a family of around 14 transferrin receptors, which has been proposed to allow binding to transferrin from different mammalian hosts. However, we find that a single receptor can bind transferrin from a broad range of mammals, indicating that receptor variation is unlikely to be necessary for promiscuity of host infection. In contrast, polymorphic sites and N-linked glycans are preferentially found in exposed positions on the receptor surface, not contacting transferrin, suggesting that transferrin receptor diversification is driven by a need for antigenic variation in the receptor to prolong survival in a host.

Journal Keywords: Parasitology; X-ray crystallography

Diamond Keywords: Sleeping Sickness

Subject Areas: Biology and Bio-materials

Instruments: I03-Macromolecular Crystallography

Added On: 25/10/2019 09:28

Discipline Tags:

Infectious Diseases Disease in the Developing World Health & Wellbeing Structural biology Life Sciences & Biotech Parasitology

Technical Tags:

Diffraction Macromolecular Crystallography (MX)