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Macroorganisation and flexibility of thylakoid membranes

DOI: 10.1042/BCJ20190080 DOI Help

Authors: Petar H. Lambrev (Biological Research Centre of the Hungarian Academy of Sciences) , Parveen Akhtar (Biological Research Centre of the Hungarian Academy of Sciences; ELI-ALPS)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Biochemical Journal , VOL 476 , PAGES 2981 - 3018

State: Published (Approved)
Published: October 2019
Diamond Proposal Number(s): 15094 , 17698 , 19120

Open Access Open Access

Abstract: The light reactions of photosynthesis are hosted and regulated by the chloroplast thylakoid membrane (TM) — the central structural component of the photosynthetic apparatus of plants and algae. The two-dimensional and three-dimensional arrangement of the lipid–protein assemblies, aka macroorganisation, and its dynamic responses to the fluctuating physiological environment, aka flexibility, are the subject of this review. An emphasis is given on the information obtainable by spectroscopic approaches, especially circular dichroism (CD). We briefly summarise the current knowledge of the composition and three-dimensional architecture of the granal TMs in plants and the supramolecular organisation of Photosystem II and light-harvesting complex II therein. We next acquaint the non-specialist reader with the fundamentals of CD spectroscopy, recent advances such as anisotropic CD, and applications for studying the structure and macroorganisation of photosynthetic complexes and membranes. Special attention is given to the structural and functional flexibility of light-harvesting complex II in vitro as revealed by CD and fluorescence spectroscopy. We give an account of the dynamic changes in membrane macroorganisation associated with the light-adaptation of the photosynthetic apparatus and the regulation of the excitation energy flow by state transitions and non-photochemical quenching.

Journal Keywords: chloroplasts; circular dichroism; light-harvesting complex II; non-photochemical quenching; photosynthetic membranes

Subject Areas: Biology and Bio-materials, Chemistry


Instruments: B23-Circular Dichroism

Documents:
bj5hg5grr.pdf