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Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans
DOI:
10.1107/S1744309110029714
PMID:
20823533
Authors:
Keisuke
Ito
(The University of Tokyo; University of Shizuoka)
,
Sohei
Ito
(University of Shizuoka)
,
Tatsuro
Shimamura
(Japan Science and Technology Agency; Kyoto University)
,
Yasuaki
Kawarasaki
(University of Shizuoka)
,
Keiko
Abe
(The University of Tokyo)
,
Takumi
Misaka
(The University of Tokyo)
,
Takuya
Kobayashi
(Japan Science and Technology Agency; Kyoto University)
,
So
Iwata
(Diamond Light Source; Imperial College London; Japan Science and Technology Agency; Kyoto University)
Co-authored by industrial partner:
No
Type:
Journal Paper
Journal:
Acta Crystallographica Section F Structural Biology And Crystallization Communications
, VOL 66 (9)
, PAGES 1086-1088
State:
Published (Approved)
Published:
September 2010
Diamond Proposal Number(s):
316
Abstract: Glucansucrases encoded by Streptococcus mutans play essential roles in the synthesis of sticky dental plaques. Based on amino-acid sequence similarity, glucansucrases are classified as members of glycoside hydrolase family 70 (GH 70). Data on the crystal structure of GH 70 glucansucrases have yet to be reported. Here, the GH 70 glucansucrase GTF-SI from S. mutans was overexpressed in Escherichia coli strain BL21 (DE3), purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. Orthorhombic GTF-SI crystals belonging to space group P2(1)2(1)2 were obtained. A diffraction data set was collected to 2.1 A resolution.
Diamond Keywords: Bacteria
Subject Areas:
Biology and Bio-materials
Instruments:
I02-Macromolecular Crystallography
,
I03-Macromolecular Crystallography
,
I04-Macromolecular Crystallography
Added On:
29/09/2010 09:22
Discipline Tags:
Dentistry
Health & Wellbeing
Structural biology
Life Sciences & Biotech
Technical Tags: