Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans

DOI: 10.1107/S1744309110029714
PMID: 20823533

Authors: Keisuke Ito (The University of Tokyo; University of Shizuoka) , Sohei Ito (University of Shizuoka) , Tatsuro Shimamura (Japan Science and Technology Agency; Kyoto University) , Yasuaki Kawarasaki (University of Shizuoka) , Keiko Abe (The University of Tokyo) , Takumi Misaka (The University of Tokyo) , Takuya Kobayashi (Japan Science and Technology Agency; Kyoto University) , So Iwata (Diamond Light Source; Imperial College London; Japan Science and Technology Agency; Kyoto University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Acta Crystallographica Section F Structural Biology And Crystallization Communications , VOL 66 (9) , PAGES 1086-1088

State: Published (Approved)
Published: January 2010
Diamond Proposal Number(s): 316

Abstract: Glucansucrases encoded by Streptococcus mutans play essential roles in the synthesis of sticky dental plaques. Based on amino-acid sequence similarity, glucansucrases are classified as members of glycoside hydrolase family 70 (GH 70). Data on the crystal structure of GH 70 glucansucrases have yet to be reported. Here, the GH 70 glucansucrase GTF-SI from S. mutans was overexpressed in Escherichia coli strain BL21 (DE3), purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. Orthorhombic GTF-SI crystals belonging to space group P2(1)2(1)2 were obtained. A diffraction data set was collected to 2.1 A resolution.

Subject Areas: Biology and Bio-materials


Instruments: I02-Macromolecular Crystallography , I03-Macromolecular Crystallography , I04-Macromolecular Crystallography

Added On: 29/09/2010 09:22

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