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Specificity of Polysaccharide Use in Intestinal Bacteroides Species Determines Diet-Induced Microbiota Alterations

DOI: 10.1016/j.cell.2010.05.005 DOI Help
PMID: 20603004 PMID Help

Authors: Erica Sonnenburg (Stanford University School of Medicine) , Hongjun Zheng (Newcastle University) , Payal Joglekar (Stanford University School of Medicine) , Steven Higginbottom (Stanford University School of Medicine) , Susan Firbank (Institute for Cell and Molecular Biosciences, Newcastle University) , David Bolam (Newcastle University) , Justin Sonnenburg (Stanford University School of Medicine)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Cell , VOL 141 (7) , PAGES 1241-1252

State: Published (Approved)
Published: June 2010

Abstract: The intestinal microbiota impacts many facets of human health and is associated with human diseases. Diet impacts microbiota composition, yet mechanisms that link dietary changes to microbiota alterations remain ill-defined. Here we elucidate the basis of Bacteroides proliferation in response to fructans, a class of fructose-based dietary polysaccharides. Structural and genetic analysis disclosed a fructose-binding, hybrid two-component signaling sensor that controls the fructan utilization locus in Bacteroides thetaiotaomicron. Gene content of this locus differs among Bacteroides species and dictates the specificity and breadth of utilizable fructans. BT1760, an extracellular β2-6 endo-fructanase, distinguishes B. thetaiotaomicron genetically and functionally, and enables the use of the β2-6-linked fructan levan. The genetic and functional differences between Bacteroides species are predictive of in vivo competitiveness in the presence of dietary fructans. Gene sequences that distinguish species' metabolic capacity serve as potential biomarkers in microbiomic datasets to enable rational manipulation of the microbiota via diet.

Journal Keywords: Bacteroides; Diet; Fructans; Germ-Free; Intestines; Inulin; Metagenome; Mice; Models; Molecular; Polysaccharides; Transcription; Genetic; Up-Regulation

Subject Areas: Biology and Bio-materials


Instruments: I02-Macromolecular Crystallography , I03-Macromolecular Crystallography , I04-Macromolecular Crystallography

Added On: 29/09/2010 09:58

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