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Catalytic and biophysical investigation of rhodium hydroformylase

DOI: 10.1039/C9CY01679A DOI Help

Authors: Hasan T. Imam (University of St Andrews; Queen's University Belfast) , Amanda G. Jarvis (University of Edinburgh) , Veronica Celorrio (Diamond Light Source) , Irshad Baig (University of St Andrews) , Christopher C. R. Allen (Queen's University Belfast) , Andrew C. Marr (Queen's University Belfast) , Paul C. J. Kamer (Leibniz Institute for Catalysis)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Catalysis Science & Technology , VOL 9 , PAGES 6428 - 6437

State: Published (Approved)
Published: November 2019
Diamond Proposal Number(s): 19850

Abstract: Rh-Containing artificial metalloenzymes based on two mutants of sterol carrier protein_2L (SCP_2L) have been shown to act as hydroformylases, exhibiting significant activity and unexpectedly high selectivity in the hydroformylation of a range of alkenes. Here we report modifications of the catalyst performance by site directed mutagenesis, and studies of the biophysical properties of these catalysts. Catalysts were prepared based on single methionine mutants of SCP_2L for hydroformylation studies. Multinuclear (1H, & 13C), multi-dimensional (2D) solution NMR spectroscopy, EXAFS and XANES were employed to probe the structure. Biophysical studies using 2D [1H 13C] HSQC NMR spectroscopy of 13C-methyl methionine labeled catalysts were used to investigate changes in protein conformation. Hydroformylation studies employing the methionine mutants as catalysts revealed the significant effects of mutation on hydroformylation activity. Among the methionine mutant catalysts M1A of V83C and A100C, and M112A of V83C exhibited significantly higher activity than their parent proteins with improved selectivity. A metal binding role for methionine was suggested by EXAFS and XANES data on selenomethionine variants.

Subject Areas: Chemistry

Instruments: B18-Core EXAFS

Other Facilities: UK Catalysis Hub

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