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In situ characterization of nanoscale strains in loaded whole joints via synchrotron X-ray tomography

DOI: 10.1038/s41551-019-0477-1 DOI Help

Authors: Kamel Madi (Research Complex at Harwell; 3Dmagination Ltd) , Katherine A. Staines (Edinburgh Napier University) , Brian K. Bay (Oregon State University) , Behzad Javaheri (Royal Veterinary College) , Hua Geng (Research Complex at Harwell; The University of Manchester) , Andrew J. Bodey (Diamond Light Source) , Sarah Cartmell (The University of Manchester) , Andrew A. Pitsillides (Royal Veterinary College) , Peter D. Lee (Research Complex at Harwell; University College London)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Biomedical Engineering , VOL 2

State: Published (Approved)
Published: November 2019
Diamond Proposal Number(s): 13237 , 11076 , 5003

Abstract: Imaging techniques for quantifying changes in the hierarchical structure of deforming joints are constrained by destructive sample treatments, sample-size restrictions and lengthy scan times. Here, we report the use of fast low-dose pink-beam synchrotron X-ray tomography in combination with mechanical loading at nanometric precision for in situ imaging, at resolutions below 100 nm, of the mechanical strain in intact untreated joints under physiologically realistic conditions. We show that in young, older and osteoarthritic mice, hierarchical changes in tissue structure and mechanical behaviour can be simultaneously visualized, and that the tissue structure at the cellular level correlates with the mechanical performance of the whole joint. We also use the tomographic approach to study the colocalization of tissue strains to specific chondrocyte lacunar organizations within intact loaded joints and to explore the role of calcified-cartilage stiffness on the biomechanics of healthy and pathological joints.

Journal Keywords: Biomedical engineering; Bone quality and biomechanics; Imaging techniques; Osteoarthritis

Diamond Keywords: Osteoarthritis; Bone

Subject Areas: Biology and Bio-materials

Instruments: I13-2-Diamond Manchester Imaging

Added On: 26/11/2019 09:27

Discipline Tags:

Life Sciences & Biotech Health & Wellbeing Non-Communicable Diseases

Technical Tags:

Imaging Tomography