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Phase-contrast 3D tomography of HeLa cells grown in PLLA polymer electrospun scaffolds using synchrotron X-rays

DOI: 10.1107/S1600577519015583 DOI Help

Authors: A. Bhartiya (University College London; Research Complex at Harwell) , K. Madi (3DMagination Ltd) , C. M. Disney (University of Manchester) , L. Courtois (3DMagination Ltd) , A. Jupe (The University of Buckingham) , F. Zhang (University College London; Research Complex at Harwell; Southern University of Science and Technology) , A. J. Bodey (Diamond Light Source) , P. Lee (University College London) , C. Rau (Diamond Light Source) , I. K. Robinson (University College London; Research Complex at Harwell; Brookhaven National Laboratory) , M. Yusuf (University College London (UCL); Research Complex at Harwell; Aga Khan University)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Synchrotron Radiation , VOL 27 , PAGES 158 - 163

State: Published (Approved)
Published: January 2020
Diamond Proposal Number(s): 14187

Abstract: Advanced imaging is useful for understanding the three-dimensional (3D) growth of cells. X-ray tomography serves as a powerful noninvasive, nondestructive technique that can fulfill these purposes by providing information about cell growth within 3D platforms. There are a limited number of studies taking advantage of synchrotron X-rays, which provides a large field of view and suitable resolution to image cells within specific biomaterials. In this study, X-ray synchrotron radiation microtomography at Diamond Light Source and advanced image processing were used to investigate cellular infiltration of HeLa cells within poly L-lactide (PLLA) scaffolds. This study demonstrates that synchrotron X-rays using phase contrast is a useful method to understand the 3D growth of cells in PLLA electrospun scaffolds. Two different fiber diameter (2 and 4 µm) scaffolds with different pore sizes, grown over 2, 5 and 8 days in vitro, were examined for infiltration and cell connectivity. After performing visualization by segmentation of the cells from the fibers, the results clearly show deeper cell growth and higher cellular interconnectivity in the 4 µm fiber diameter scaffold. This indicates the potential for using such 3D technology to study cell–scaffold interactions for future medical use.

Journal Keywords: 3D culture; electrospun polymer scaffold; X-ray CT; synchrotron; image analysis; Avizo

Subject Areas: Technique Development, Biology and Bio-materials

Instruments: I13-2-Diamond Manchester Imaging

Added On: 07/01/2020 11:09

Discipline Tags:

Life Sciences & Biotech Technique Development - Life Sciences & Biotech

Technical Tags:

Imaging Tomography