Publication

Article Metrics

Citations


Online attention

Re-evaluation of human BDCA-2+ DC during acute sterile skin inflammation

DOI: 10.1084/jem.20190811 DOI Help

Authors: Yi-ling Chen (Weatherall Institute of Molecular Medicine, University of Oxford) , Tomas Gomes (Open Targets) , Clare S. Hardman (Weatherall Institute of Molecular Medicine, University of Oxford) , Felipe A. Vieira Braga (Wellcome Sanger Institute; Open Targets) , Danuta Gutowska-owsiak (Weatherall Institute of Molecular Medicine, University of Oxford; University of Gdańsk; Medical University of Gdańsk) , Maryam Salimi (Weatherall Institute of Molecular Medicine, University of Oxford) , Nicki Gray (Weatherall Institute of Molecular Medicine, University of Oxford) , David A. Duncan (Diamond Light Source) , Gary Reynolds (Institute of Cellular Medicine) , David Johnson (John Radcliffe Hospital, Oxford University Hospitals NHS Foundation Trust) , Mariolina Salio (Weatherall Institute of Molecular Medicine, University of Oxford) , Vincenzo Cerundolo (Weatherall Institute of Molecular Medicine, University of Oxford) , Jillian L. Barlow (MRC Laboratory of Molecular Biology) , Andrew N. J. Mckenzie (MRC Laboratory of Molecular Biology) , Sarah A. Teichmann (Wellcome Sanger Institute; European Bioinformatics Institute (EMBLEBI); University of Cambridge) , Muzlifah Haniffa (Wellcome Sanger Institute; Institute of Cellular Medicine; Newcastle Hospitals NHS Foundation Trust) , Graham Ogg (Weatherall Institute of Molecular Medicine, University of Oxford)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: The Journal Of Experimental Medicine , VOL 217

State: Published (Approved)
Published: March 2020

Abstract: Plasmacytoid dendritic cells (pDCs) produce type I interferon (IFN-I) and are traditionally defined as being BDCA-2+CD123+. pDCs are not readily detectable in healthy human skin, but have been suggested to accumulate in wounds. Here, we describe a CD1a-bearing BDCA-2+CD123int DC subset that rapidly infiltrates human skin wounds and comprises a major DC population. Using single-cell RNA sequencing, we show that these cells are largely activated DCs acquiring features compatible with lymph node homing and antigen presentation, but unexpectedly express both BDCA-2 and CD123, potentially mimicking pDCs. Furthermore, a third BDCA-2–expressing population, Axl+Siglec-6+ DCs (ASDC), was also found to infiltrate human skin during wounding. These data demonstrate early skin infiltration of a previously unrecognized CD123intBDCA-2+CD1a+ DC subset during acute sterile inflammation, and prompt a re-evaluation of previously ascribed pDC involvement in skin disease.

Subject Areas: Biology and Bio-materials


Technical Areas: