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Structural basis of cycloaddition in biosynthesis of iboga and aspidosperma alkaloids

DOI: 10.1038/s41589-019-0460-x DOI Help

Authors: Lorenzo Caputi (Max Planck Institute of Chemical Ecology) , Jakob Franke (Leibniz University Hannover) , Kate Bussey (John Innes Centre) , Scott C. Farrow (John Innes Centre) , Ivo Jose Curcino Vieira (Laboratorio de Ciencias Quimicas-UENF-Campos dos Goytacazes-RJ) , Clare E. M. Stevenson (John Innes Centre) , David N. Lawson (John Innes Centre) , Sarah E. O’connor (Max Planck Institute of Chemical Ecology)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Nature Chemical Biology , VOL 23

State: Published (Approved)
Published: February 2020
Diamond Proposal Number(s): 13467

Abstract: Cycloaddition reactions generate chemical complexity in a single step. Here we report the crystal structures of three homologous plant-derived cyclases involved in the biosynthesis of iboga and aspidosperma alkaloids. These enzymes act on the same substrate, named angryline, to generate three distinct scaffolds. Mutational analysis reveals how these highly similar enzymes control regio- and stereo-selectivity.

Journal Keywords: Biosynthesis; Enzyme mechanisms; Enzymes; X-ray crystallography

Diamond Keywords: Enzymes

Subject Areas: Biology and Bio-materials, Chemistry

Instruments: I03-Macromolecular Crystallography

Added On: 25/02/2020 14:46

Discipline Tags:

Biochemistry Catalysis Chemistry Structural biology Organic Chemistry Life Sciences & Biotech

Technical Tags:

Diffraction Macromolecular Crystallography (MX)