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The HARE chip for efficient time-resolved serial synchrotron crystallography

DOI: 10.1107/S1600577520000685 DOI Help

Authors: Pedram Mehrabi (Max-Planck-Institute for Structure and Dynamics of Matter) , Henrike Mueller-werkmeister (Max-Planck-Institute for Structure and Dynamics of Matter; University of Potsdam) , Jan-philipp Leimkohl (Max-Planck-Institute for Structure and Dynamics of Matter) , Hendrik Schikora (Max-Planck-Institute for Structure and Dynamics of Matter) , Jelena Ninkovic (Halbleiterlabor der Max-Planck-Gesellschaft) , Silvia Krivokuca (Halbleiterlabor der Max-Planck-Gesellschaft) , Ladislav Andriček (Halbleiterlabor der Max-Planck-Gesellschaft) , Sascha W. Epp (Max-Planck-Institute for Structure and Dynamics of Matter) , Darren A. Sherrell (Diamond Light Source) , Robin L. Owen (Diamond Light Source) , Arwen R. Pearson (Universität Hamburg) , Friedjof Tellkamp (Max-Planck-Institute for Structure and Dynamics of Matter) , Eike Schulz (Max-Planck-Institute for Structure and Dynamics of Matter) , R. J. Dwayne Miller (Max-Planck-Institute for Structure and Dynamics of Matter; Universität Hamburg; University of Toronto)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Journal Of Synchrotron Radiation , VOL 27 , PAGES 360 - 370

State: Published (Approved)
Published: March 2020

Open Access Open Access

Abstract: Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided.

Journal Keywords: data collection; sample delivery; fixed-target serial synchrotron crystallography; time-resolved crystallography; HARE; LAMA

Subject Areas: Technique Development

Facility: EMBL beamline P14 at PETRA III

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