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Industrial cryo-EM facility setup and management

DOI: 10.1107/S2059798320002223 DOI Help

Authors: Kasim Sader (Thermo Fisher Scientific) , Rishi Matadeen (Thermo Fisher Scientific; Diamond Light Source) , Pablo Castro Hartmann (Thermo Fisher Scientific) , Tor Halsan (Thermo Fisher Scientific) , Chris Schlichten (Thermo Fisher Scientific)
Co-authored by industrial partner: Yes

Type: Journal Paper
Journal: Acta Crystallographica Section D Structural Biology , VOL 76 , PAGES 313 - 325

State: Published (Approved)
Published: April 2020

Open Access Open Access

Abstract: Cryo-electron microscopy (cryo-EM) has rapidly expanded with the introduction of direct electron detectors, improved image-processing software and automated image acquisition. Its recent adoption by industry, particularly in structure-based drug design, creates new requirements in terms of reliability, reproducibility and throughput. In 2016, Thermo Fisher Scientific (then FEI) partnered with the Medical Research Council Laboratory of Molecular Biology, the University of Cambridge Nanoscience Centre and five pharmaceutical companies [Astex Pharmaceuticals, AstraZeneca, GSK, Sosei Heptares and Union Chimique Belge (UCB)] to form the Cambridge Pharmaceutical Cryo-EM Consortium to share the risks of exploring cryo-EM for early-stage drug discovery. The Consortium expanded with a second Themo Scientific Krios Cryo-EM at the University of Cambridge Department of Materials Science and Metallurgy. Several Consortium members have set up in-house facilities, and a full service cryo-EM facility with Krios and Glacios has been created with the Electron Bio-Imaging Centre for Industry (eBIC for Industry) at Diamond Light Source (DLS), UK. This paper will cover the lessons learned during the setting up of these facilities, including two Consortium Krios microscopes and preparation laboratories, several Glacios microscopes at Consortium member sites, and a Krios and Glacios at eBIC for Industry, regarding site evaluation and selection for high-resolution cryo-EM microscopes, the installation process, scheduling, the operation and maintenance of the microscopes and preparation laboratories, and image processing.

Journal Keywords: cryo-EM; facility setup; facility management; industry

Subject Areas: Technique Development

Technical Areas:

Added On: 15/04/2020 08:56


Discipline Tags:

Technique Development - Life Sciences & Biotech Life Sciences & Biotech

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