Detection and imaging of gadolinium accumulation in human bone tissue by micro- and submicro-XRF

DOI: 10.1038/s41598-020-63325-9

Authors: Anna Turyanskaya (TU Wien) , Mirjam Rauwolf (TU Wien) , Vanessa Pichler (TU Wien) , Rolf Simon (ANKA Synchrotron Radiation Source) , Manfred Burghammer (European Synchrotron Radiation Facility) , Oliver J. L. Fox (Diamond Light Source) , Kawal Sawhney (Diamond Light Source) , Jochen G. Hofstaetter (1st Medical Department Hanusch Hospital; Orthopaedic Hospital Vienna-Speising) , Andreas Roschger (1st Medical Department Hanusch Hospital; Paris-Lodron-University of Salzburg) , Paul Roschger (1st Medical Department Hanusch Hospital) , Peter Wobrauschek (TU Wien) , Christina Streli (TU Wien)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Scientific Reports , VOL 10

State: Published (Approved)
Published: April 2020
Diamond Proposal Number(s): 16260

Abstract: Gadolinium-based contrast agents (GBCAs) are frequently used in patients undergoing magnetic resonance imaging. In GBCAs gadolinium (Gd) is present in a bound chelated form. Gadolinium is a rare-earth element, which is normally not present in human body. Though the blood elimination half-life of contrast agents is about 90 minutes, recent studies demonstrated that some tissues retain gadolinium, which might further pose a health threat due to toxic effects of free gadolinium. It is known that the bone tissue can serve as a gadolinium depot, but so far only bulk measurements were performed. Here we present a summary of experiments in which for the first time we mapped gadolinium in bone biopsy from a male patient with idiopathic osteoporosis (without indication of renal impairment), who received MRI 8 months prior to biopsy. In our studies performed by means of synchrotron radiation induced micro- and submicro-X-ray fluorescence spectroscopy (SR-XRF), gadolinium was detected in human cortical bone tissue. The distribution of gadolinium displays a specific accumulation pattern. Correlation of elemental maps obtained at ANKA synchrotron with qBEI images (quantitative backscattered electron imaging) allowed assignment of Gd structures to the histological bone structures. Follow-up beamtimes at ESRF and Diamond Light Source using submicro-SR-XRF allowed resolving thin Gd structures in cortical bone, as well as correlating them with calcium and zinc.

Diamond Keywords: Bone

Subject Areas: Biology and Bio-materials


Instruments: B16-Test Beamline

Other Facilities: ANKA; ESRF

Added On: 15/04/2020 14:24

Documents:
s41598-020-63325-9.pdf

Discipline Tags:

Osteoporosis Non-Communicable Diseases Health & Wellbeing Life Sciences & Biotech

Technical Tags:

Imaging X-ray Fluorescence (XRF)