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Differential impact on motility and biofilm dispersal of closely related phosphodiesterases in Pseudomonas aeruginosa

DOI: 10.1038/s41598-020-63008-5 DOI Help

Authors: Yu-ming Cai (University of Southampton) , Andrew Hutchin (Université Libre de Bruxelles; University of Southampton; Diamond Light Source) , Jack Craddock (University of Southampton) , Martin A. Walsh (Diamond Light Source; Research Complex at Harwell) , Jeremy S. Webb (University of Southampton) , Ivo Tews (University of Southampton)
Co-authored by industrial partner: No

Type: Journal Paper
Journal: Scientific Reports , VOL 10

State: Published (Approved)
Published: April 2020

Open Access Open Access

Abstract: In Pseudomonas aeruginosa, the transition between planktonic and biofilm lifestyles is modulated by the intracellular secondary messenger cyclic dimeric-GMP (c-di-GMP) in response to environmental conditions. Here, we used gene deletions to investigate how the environmental stimulus nitric oxide (NO) is linked to biofilm dispersal, focusing on biofilm dispersal phenotype from proteins containing putative c-di-GMP turnover and Per-Arnt-Sim (PAS) sensory domains. We document opposed physiological roles for the genes ΔrbdA and Δpa2072 that encode proteins with identical domain structure: while ΔrbdA showed elevated c-di-GMP levels, restricted motility and promoted biofilm formation, c-di-GMP levels were decreased in Δpa2072, and biofilm formation was inhibited, compared to wild type. A second pair of genes, ΔfimX and ΔdipA, were selected on the basis of predicted impaired c-di-GMP turnover function: ΔfimX showed increased, ΔdipA decreased NO induced biofilm dispersal, and the genes effected different types of motility, with reduced twitching for ΔfimX and reduced swimming for ΔdipA. For all four deletion mutants we find that NO-induced biomass reduction correlates with increased NO-driven swarming, underlining a significant role for this motility in biofilm dispersal. Hence P. aeruginosa is able to differentiate c-di-GMP output using structurally highly related proteins that can contain degenerate c-di-GMP turnover domains.

Journal Keywords: Biofilms; Microbial communities; Microbiology; Mutation

Subject Areas: Biology and Bio-materials


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s41598-020-63008-5.pdf